(Linear) (Six-base) MAPSORT of: KIEE6180.seq  Check: 334  from: 1  to: 381



KIEE6180.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:28  ..

ApoI r'AATT_y

Cuts at:      0      62     381 
   Size:         62     319
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     315     348     381 
   Size:        315      33      33

  Fragments arranged by size: 

                 315      33      33
 
BclI T'GATC_A

Cuts at:      0     351     381 
   Size:        351      30
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      96     381 
   Size:         96     285
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      18     381 
   Size:         18     363
 
BsaBI GATnn'nnATC

Cuts at:      0     161     381 
   Size:        161     220
 
BsaHI Gr'CG_yC

Cuts at:      0     317     381 
   Size:        317      64
 
BseYI C'CCAG_C

Cuts at:      0      68     381 
   Size:         68     313
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      56     381 
   Size:         56     325
 
BsiEI CG_ry'CG

Cuts at:      0     314     381 
   Size:        314      67
 
BsmI GAATG_Cn'

Cuts at:      0     141     381 
   Size:        141     240
 
BspHI T'CATG_A

Cuts at:      0     335     381 
   Size:        335      46
 
BtgI C'CryG_G

Cuts at:      0      97     224     237     381 
   Size:         97     127      13     144

  Fragments arranged by size: 

                 144     127      97      13
 
BtsI GCAGTG_nn'

Cuts at:      0     146     381 
   Size:        146     235
 
EaeI y'GGCC_r

Cuts at:      0     240     381 
   Size:        240     141
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      96     381 
   Size:         96     285
 
EcoO109I rG'GnC_Cy

Cuts at:      0     283     381 
   Size:        283      98
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     301     381 
   Size:        301      80
 
MscI TGG'CCA

Cuts at:      0     242     381 
   Size:        242     139
 
MslI CAynn'nnrTG

Cuts at:      0     102     201     381 
   Size:        102      99     180

  Fragments arranged by size: 

                 180     102      99
 
NcoI C'CATG_G

Cuts at:      0      97     224     237     381 
   Size:         97     127      13     144

  Fragments arranged by size: 

                 144     127      97      13
 
PvuI CG_AT'CG

Cuts at:      0     314     381 
   Size:        314      67
 
SspI AAT'ATT

Cuts at:      0     372     381 
   Size:        372       9
 
StyI C'CwwG_G

Cuts at:      0      97     224     237     381 
   Size:         97     127      13     144

  Fragments arranged by size: 

                 144     127      97      13
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      76     381 
   Size:         76     305
 
XmnI GAAnn'nnTTC

Cuts at:      0      63     381 
   Size:         63     318
 

 Enzymes that do cut:

     ApoI     BaeI     BclI     BpmI     BsaI    BsaBI    BsaHI    BseYI
     BsgI    BsiEI     BsmI    BspHI     BtgI     BtsI     EaeI  Eco57MI
 EcoO109I     MmeI     MscI     MslI     NcoI     PvuI     SspI     StyI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaWI    BsaXI    BseRI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     ZraI