(Linear) (Six-base) MAPSORT of: KIEE6179.seq  Check: 2520  from: 1  to: 357



KIEE6179.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:28  ..

BbsI GAAGACnn'nnnn_

Cuts at:      0     152     353     357 
   Size:        152     201       4

  Fragments arranged by size: 

                 201     152       4
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     117     357 
   Size:        117     240
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     291     357 
   Size:        291      66
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     357 
   Size:        286      71
 
BsaHI Gr'CG_yC

Cuts at:      0     236     296     357 
   Size:        236      60      61

  Fragments arranged by size: 

                 236      61      60
 
BseYI C'CCAG_C

Cuts at:      0      63     357 
   Size:         63     294
 
BsiHKAI G_wGCw'C

Cuts at:      0     287     357 
   Size:        287      70
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      29     357 
   Size:         29     328
 
Bsp1286I G_dGCh'C

Cuts at:      0     287     357 
   Size:        287      70
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     108     357 
   Size:        108     249
 
BstYI r'GATC_y

Cuts at:      0     269     357 
   Size:        269      88
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0       6     192     357 
   Size:          6     186     165

  Fragments arranged by size: 

                 186     165       6
 
BtsI GCAGTG_nn'

Cuts at:      0      32     338     357 
   Size:         32     306      19

  Fragments arranged by size: 

                 306      32      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     357 
   Size:        286      71
 
FspI TGC'GCA

Cuts at:      0     319     357 
   Size:        319      38
 
MslI CAynn'nnrTG

Cuts at:      0      25     290     357 
   Size:         25     265      67

  Fragments arranged by size: 

                 265      67      25
 
MspA1I CmG'CkG

Cuts at:      0     259     357 
   Size:        259      98
 
NdeI CA'TA_TG

Cuts at:      0     291     357 
   Size:        291      66
 
NspI r_CATG'y

Cuts at:      0     229     336     357 
   Size:        229     107      21

  Fragments arranged by size: 

                 229     107      21
 
PfoI T'CCnGG_A

Cuts at:      0     263     357 
   Size:        263      94
 
SphI G_CATG'C

Cuts at:      0     229     357 
   Size:        229     128
 
StyI C'CwwG_G

Cuts at:      0     278     302     357 
   Size:        278      24      55

  Fragments arranged by size: 

                 278      55      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     302     357 
   Size:        302      55
 
TatI w'GTAC_w

Cuts at:      0     203     357 
   Size:        203     154
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     232     264     357 
   Size:        232      32      93

  Fragments arranged by size: 

                 232      93      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     108     357 
   Size:        108     249
 

 Enzymes that do cut:

     BbsI    BciVI     BplI     BpmI    BsaHI    BseYI  BsiHKAI    BsmBI
 Bsp1286I    BstXI    BstYI    BtgZI     BtsI  Eco57MI     FspI     MslI
   MspA1I     NdeI     NspI     PfoI     SphI     StyI    TaqII     TatI
     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI