(Linear) (Six-base) MAPSORT of: KIEE6178.seq  Check: 9017  from: 1  to: 354



KIEE6178.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:28  ..

AatII G_ACGT'C

Cuts at:      0     317     354 
   Size:        317      37
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     354 
   Size:        118     236
 
AlwNI CAG_nnn'CTG

Cuts at:      0      33     354 
   Size:         33     321
 
ApoI r'AATT_y

Cuts at:      0     160     354 
   Size:        160     194
 
BanI G'GyrC_C

Cuts at:      0     233     354 
   Size:        233     121
 
BanII G_rGCy'C

Cuts at:      0     193     259     354 
   Size:        193      66      95

  Fragments arranged by size: 

                 193      95      66
 
BbeI G_GCGC'C

Cuts at:      0     237     354 
   Size:        237     117
 
BbvCI CC'TCA_GC

Cuts at:      0     212     354 
   Size:        212     142
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      31     354 
   Size:         31     323
 
Bpu10I CC'TnA_GC

Cuts at:      0     212     354 
   Size:        212     142
 
BsaHI Gr'CG_yC

Cuts at:      0     234     314     354 
   Size:        234      80      40

  Fragments arranged by size: 

                 234      80      40
 
BsaWI w'CCGG_w

Cuts at:      0     341     354 
   Size:        341      13
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     127     354 
   Size:        127     227
 
BseYI C'CCAG_C

Cuts at:      0     260     290     354 
   Size:        260      30      64

  Fragments arranged by size: 

                 260      64      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     354 
   Size:        262      92
 
BsiHKAI G_wGCw'C

Cuts at:      0     158     259     354 
   Size:        158     101      95

  Fragments arranged by size: 

                 158     101      95
 
Bsp1286I G_dGCh'C

Cuts at:      0     158     193     259     354 
   Size:        158      35      66      95

  Fragments arranged by size: 

                 158      95      66      35
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      33     249     308     354 
   Size:         33     216      59      46

  Fragments arranged by size: 

                 216      59      46      33
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     298     354 
   Size:        298      56
 
BtgI C'CryG_G

Cuts at:      0     185     354 
   Size:        185     169
 
EaeI y'GGCC_r

Cuts at:      0       2     182     354 
   Size:          2     180     172

  Fragments arranged by size: 

                 180     172       2
 
EcoICRI GAG'CTC

Cuts at:      0     257     354 
   Size:        257      97
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     354 
   Size:        118     236
 
EcoO109I rG'GnC_Cy

Cuts at:      0     320     354 
   Size:        320      34
 
EcoRI G'AATT_C

Cuts at:      0     160     354 
   Size:        160     194
 
HaeII r_GCGC'y

Cuts at:      0     237     354 
   Size:        237     117
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     266     298     354 
   Size:        266      32      56

  Fragments arranged by size: 

                 266      56      32
 
KasI G'GCGC_C

Cuts at:      0     233     354 
   Size:        233     121
 
MscI TGG'CCA

Cuts at:      0       4     354 
   Size:          4     350
 
MspA1I CmG'CkG

Cuts at:      0      55     354 
   Size:         55     299
 
NarI GG'CG_CC

Cuts at:      0     234     354 
   Size:        234     120
 
NcoI C'CATG_G

Cuts at:      0     185     354 
   Size:        185     169
 
NspI r_CATG'y

Cuts at:      0     252     354 
   Size:        252     102
 
PasI CC'CwG_GG

Cuts at:      0     195     354 
   Size:        195     159
 
PvuII CAG'CTG

Cuts at:      0      55     354 
   Size:         55     299
 
SacI G_AGCT'C

Cuts at:      0     259     354 
   Size:        259      95
 
SfoI GGC'GCC

Cuts at:      0     235     354 
   Size:        235     119
 
SphI G_CATG'C

Cuts at:      0     252     354 
   Size:        252     102
 
StyI C'CwwG_G

Cuts at:      0      15     185     354 
   Size:         15     170     169

  Fragments arranged by size: 

                 170     169      15
 
ZraI GAC'GTC

Cuts at:      0     315     354 
   Size:        315      39
 

 Enzymes that do cut:

    AatII     AcuI    AlwNI     ApoI     BanI    BanII     BbeI    BbvCI
    BciVI   Bpu10I    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I   BstAPI    BstXI     BtgI     EaeI  EcoICRI  Eco57MI EcoO109I
    EcoRI    HaeII    Hin4I     KasI     MscI   MspA1I     NarI     NcoI
     NspI     PasI    PvuII     SacI     SfoI     SphI     StyI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbsI     BcgI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaXI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI    EcoNI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MslI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI