(Linear) (Six-base) MAPSORT of: KIEE6177.seq  Check: 1785  from: 1  to: 324



KIEE6177.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       3     324 
   Size:          3     321
 
ApoI r'AATT_y

Cuts at:      0     280     295     324 
   Size:        280      15      29

  Fragments arranged by size: 

                 280      29      15
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     232     324 
   Size:        191      41      92

  Fragments arranged by size: 

                 191      92      41
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     324 
   Size:        217     107
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     164     194     324 
   Size:        164      30     130

  Fragments arranged by size: 

                 164     130      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      18     324 
   Size:         18     306
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      54     324 
   Size:         54     270
 
BstYI r'GATC_y

Cuts at:      0     288     324 
   Size:        288      36
 
BtsI GCAGTG_nn'

Cuts at:      0      92     324 
   Size:         92     232
 
EarI CTCTTCn'nnn_

Cuts at:      0      22     324 
   Size:         22     302
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       3     191     232     324 
   Size:          3     188      41      92

  Fragments arranged by size: 

                 188      92      41       3
 
EcoO109I rG'GnC_Cy

Cuts at:      0     175     324 
   Size:        175     149
 
PpuMI rG'GwC_Cy

Cuts at:      0     175     324 
   Size:        175     149
 
SapI GCTCTTCn'nnn_

Cuts at:      0      22     324 
   Size:         22     302
 
SmlI C'TyrA_G

Cuts at:      0     232     324 
   Size:        232      92
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      29     324 
   Size:         29     295
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      60      92     324 
   Size:         60      32     232

  Fragments arranged by size: 

                 232      60      32
 

 Enzymes that do cut:

     AcuI     ApoI     BpmI    BpuEI    BsaXI    BseRI    BsmBI    BstYI
     BtsI     EarI  Eco57MI EcoO109I    PpuMI     SapI     SmlI    TaqII
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI