(Linear) (Six-base) MAPSORT of: KIEE6175.seq  Check: 2805  from: 1  to: 333



KIEE6175.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:27  ..

ApoI r'AATT_y

Cuts at:      0     261     333 
   Size:        261      72
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     153     333 
   Size:        153     180
 
BglII A'GATC_T

Cuts at:      0     221     333 
   Size:        221     112
 
Bme1580I G_kGCm'C

Cuts at:      0     198     333 
   Size:        198     135
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      97     333 
   Size:         97     236
 
Bsp1286I G_dGCh'C

Cuts at:      0     198     333 
   Size:        198     135
 
BstBI TT'CG_AA

Cuts at:      0      51     333 
   Size:         51     282
 
BstYI r'GATC_y

Cuts at:      0     221     333 
   Size:        221     112
 
BtsI GCAGTG_nn'

Cuts at:      0     244     333 
   Size:        244      89
 
EarI CTCTTCn'nnn_

Cuts at:      0     248     333 
   Size:        248      85
 
PsiI TTA'TAA

Cuts at:      0     167     333 
   Size:        167     166
 
PstI C_TGCA'G

Cuts at:      0      75     333 
   Size:         75     258
 
SfcI C'TryA_G

Cuts at:      0      71     333 
   Size:         71     262
 

 Enzymes that do cut:

     ApoI     BbsI    BglII Bme1580I     BmrI Bsp1286I    BstBI    BstYI
     BtsI     EarI     PsiI     PstI     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI