(Linear) (Six-base) MAPSORT of: KIEE6174.seq  Check: 4243  from: 1  to: 273



KIEE6174.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:27  ..

AvaI C'yCGr_G

Cuts at:      0     137     273 
   Size:        137     136
 
BamHI G'GATC_C

Cuts at:      0      81     273 
   Size:         81     192
 
BanII G_rGCy'C

Cuts at:      0     155     212     273 
   Size:        155      57      61

  Fragments arranged by size: 

                 155      61      57
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     180     273 
   Size:        180      93
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     273 
   Size:         28     245
 
BsaWI w'CCGG_w

Cuts at:      0      37     273 
   Size:         37     236
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      64      67      82     220     273 
   Size:         64       3      15     138      53

  Fragments arranged by size: 

                 138      64      53      15       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     155     212     273 
   Size:        155      57      61

  Fragments arranged by size: 

                 155      61      57
 
Bsp1286I G_dGCh'C

Cuts at:      0     155     212     273 
   Size:        155      57      61

  Fragments arranged by size: 

                 155      61      57
 
BspEI T'CCGG_A

Cuts at:      0      37     273 
   Size:         37     236
 
BsrBI CCG'CTC

Cuts at:      0     144     273 
   Size:        144     129
 
BsrDI GCAATG_nn'

Cuts at:      0     112     237     273 
   Size:        112     125      36

  Fragments arranged by size: 

                 125     112      36
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      24     273 
   Size:         24     249
 
BstYI r'GATC_y

Cuts at:      0      42      81     273 
   Size:         42      39     192

  Fragments arranged by size: 

                 192      42      39
 
Bsu36I CC'TnA_GG

Cuts at:      0      47     273 
   Size:         47     226
 
EarI CTCTTCn'nnn_

Cuts at:      0      53      59     185     273 
   Size:         53       6     126      88

  Fragments arranged by size: 

                 126      88      53       6
 
EcoICRI GAG'CTC

Cuts at:      0     153     210     273 
   Size:        153      57      63

  Fragments arranged by size: 

                 153      63      57
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     273 
   Size:         28     245
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     154     186     273 
   Size:        154      32      87

  Fragments arranged by size: 

                 154      87      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     273 
   Size:         98     175
 
NspI r_CATG'y

Cuts at:      0     229     273 
   Size:        229      44
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     243     273 
   Size:        243      30
 
SacI G_AGCT'C

Cuts at:      0     155     212     273 
   Size:        155      57      61

  Fragments arranged by size: 

                 155      61      57
 
SmaI CCC'GGG

Cuts at:      0     139     273 
   Size:        139     134
 
SphI G_CATG'C

Cuts at:      0     229     273 
   Size:        229      44
 
XmaI C'CCGG_G

Cuts at:      0     137     273 
   Size:        137     136
 

 Enzymes that do cut:

     AvaI    BamHI    BanII    BciVI     BpmI    BsaWI    BseRI  BsiHKAI
 Bsp1286I    BspEI    BsrBI    BsrDI   BstAPI    BstYI   Bsu36I     EarI
  EcoICRI  Eco57MI    Hin4I     MmeI     NspI    PflMI     SacI     SmaI
     SphI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BanI
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI