(Linear) (Six-base) MAPSORT of: KIEE6173.seq  Check: 5051  from: 1  to: 243



KIEE6173.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:27  ..

BanI G'GyrC_C

Cuts at:      0      69     243 
   Size:         69     174
 
BstEII G'GTnAC_C

Cuts at:      0     123     243 
   Size:        123     120
 
Bsu36I CC'TnA_GG

Cuts at:      0      86     243 
   Size:         86     157
 
EcoO109I rG'GnC_Cy

Cuts at:      0      91     243 
   Size:         91     152
 
MfeI C'AATT_G

Cuts at:      0     199     243 
   Size:        199      44
 
MslI CAynn'nnrTG

Cuts at:      0      34     115     121     243 
   Size:         34      81       6     122

  Fragments arranged by size: 

                 122      81      34       6
 
PpuMI rG'GwC_Cy

Cuts at:      0      91     243 
   Size:         91     152
 
StyI C'CwwG_G

Cuts at:      0      73     243 
   Size:         73     170
 

 Enzymes that do cut:

     BanI   BstEII   Bsu36I EcoO109I     MfeI     MslI    PpuMI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI