(Linear) (Six-base) MAPSORT of: KIEE6172.seq  Check: 9819  from: 1  to: 246



KIEE6172.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     246 
   Size:        212      34
 
BanI G'GyrC_C

Cuts at:      0     147     246 
   Size:        147      99
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      81     246 
   Size:         81     165
 
BclI T'GATC_A

Cuts at:      0      41     246 
   Size:         41     205
 
BmgBI CAC'GTC

Cuts at:      0      87     246 
   Size:         87     159
 
BsmI GAATG_Cn'

Cuts at:      0     122     246 
   Size:        122     124
 
BssHII G'CGCG_C

Cuts at:      0      72     246 
   Size:         72     174
 
BstYI r'GATC_y

Cuts at:      0     205     246 
   Size:        205      41
 
EarI CTCTTCn'nnn_

Cuts at:      0     188     246 
   Size:        188      58
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     246 
   Size:        212      34
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     204     236     246 
   Size:        204      32      10

  Fragments arranged by size: 

                 204      32      10
 
MspA1I CmG'CkG

Cuts at:      0     226     246 
   Size:        226      20
 
NspI r_CATG'y

Cuts at:      0      40     246 
   Size:         40     206
 
PvuII CAG'CTG

Cuts at:      0     226     246 
   Size:        226      20
 
SfcI C'TryA_G

Cuts at:      0      48     246 
   Size:         48     198
 

 Enzymes that do cut:

     AcuI     BanI     BbsI     BclI    BmgBI     BsmI   BssHII    BstYI
     EarI  Eco57MI    Hin4I   MspA1I     NspI    PvuII     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI