(Linear) (Six-base) MAPSORT of: KIEE6170.seq  Check: 4919  from: 1  to: 207



KIEE6170.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:27  ..

AgeI A'CCGG_T

Cuts at:      0       9     207 
   Size:          9     198
 
AvaI C'yCGr_G

Cuts at:      0     104     207 
   Size:        104     103
 
BanI G'GyrC_C

Cuts at:      0       3     207 
   Size:          3     204
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      24     207 
   Size:         24     183
 
BsaWI w'CCGG_w

Cuts at:      0       9     207 
   Size:          9     198
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     130     135     207 
   Size:        130       5      72

  Fragments arranged by size: 

                 130      72       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     207 
   Size:         34     173
 
BsiEI CG_ry'CG

Cuts at:      0      42     207 
   Size:         42     165
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0       6     207 
   Size:          6     201
 
BsrBI CCG'CTC

Cuts at:      0      27     207 
   Size:         27     180
 
BsrFI r'CCGG_y

Cuts at:      0       9     207 
   Size:          9     198
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     180     207 
   Size:        180      27
 
EaeI y'GGCC_r

Cuts at:      0      39     207 
   Size:         39     168
 
EagI C'GGCC_G

Cuts at:      0      39     207 
   Size:         39     168
 
EarI CTCTTCn'nnn_

Cuts at:      0     107     131     207 
   Size:        107      24      76

  Fragments arranged by size: 

                 107      76      24
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      39     207 
   Size:         39     168
 
SgrAI Cr'CCGG_yG

Cuts at:      0       9     207 
   Size:          9     198
 

 Enzymes that do cut:

     AgeI     AvaI     BanI     BsaI    BsaWI    BseRI     BsgI    BsiEI
    BspMI    BsrBI    BsrFI    BstXI     EaeI     EagI     EarI     EciI
    SgrAI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI    BseYI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI