(Linear) (Six-base) MAPSORT of: KIEE6168.seq  Check: 3269  from: 1  to: 423



KIEE6168.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:27  ..

Acc65I G'GTAC_C

Cuts at:      0     272     423 
   Size:        272     151
 
BanI G'GyrC_C

Cuts at:      0     272     423 
   Size:        272     151
 
BbvCI CC'TCA_GC

Cuts at:      0     247     258     423 
   Size:        247      11     165

  Fragments arranged by size: 

                 247     165      11
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     283     317     423 
   Size:        283      34     106

  Fragments arranged by size: 

                 283     106      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     218     423 
   Size:        139      79     205

  Fragments arranged by size: 

                 205     139      79
 
Bpu10I CC'TnA_GC

Cuts at:      0     247     258     305     423 
   Size:        247      11      47     118

  Fragments arranged by size: 

                 247     118      47      11
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     316     344     346     374     423 
   Size:        316      28       2      28      49

  Fragments arranged by size: 

                 316      49      28      28       2
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     243     246     370     423 
   Size:        243       3     124      53

  Fragments arranged by size: 

                 243     124      53       3
 
BseYI C'CCAG_C

Cuts at:      0     335     344     397     423 
   Size:        335       9      53      26

  Fragments arranged by size: 

                 335      53      26       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     423 
   Size:        117     306
 
BsrBI CCG'CTC

Cuts at:      0     270     423 
   Size:        270     153
 
Bsu36I CC'TnA_GG

Cuts at:      0     382     423 
   Size:        382      41
 
BtgI C'CryG_G

Cuts at:      0     182     423 
   Size:        182     241
 
BtsI GCAGTG_nn'

Cuts at:      0     129     423 
   Size:        129     294
 
EaeI y'GGCC_r

Cuts at:      0     290     423 
   Size:        290     133
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     218     423 
   Size:        139      79     205

  Fragments arranged by size: 

                 205     139      79
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     292     368     423 
   Size:        292      76      55

  Fragments arranged by size: 

                 292      76      55
 
EcoO109I rG'GnC_Cy

Cuts at:      0     243     423 
   Size:        243     180
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     171     423 
   Size:        171     252
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     316     348     423 
   Size:        316      32      75

  Fragments arranged by size: 

                 316      75      32
 
KpnI G_GTAC'C

Cuts at:      0     276     423 
   Size:        276     147
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     318     423 
   Size:        318     105
 
PpuMI rG'GwC_Cy

Cuts at:      0     243     423 
   Size:        243     180
 
PshAI GACnn'nnGTC

Cuts at:      0     241     423 
   Size:        241     182
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     265     297     423 
   Size:        265      32     126

  Fragments arranged by size: 

                 265     126      32
 
TatI w'GTAC_w

Cuts at:      0      26     423 
   Size:         26     397
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     344     376     423 
   Size:        344      32      47

  Fragments arranged by size: 

                 344      47      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     285     423 
   Size:        285     138
 

 Enzymes that do cut:

   Acc65I     BanI    BbvCI     BcgI     BpmI   Bpu10I    BsaXI    BseRI
    BseYI     BsgI    BsrBI   Bsu36I     BtgI     BtsI     EaeI  Eco57MI
    EcoNI EcoO109I     FalI    Hin4I     KpnI    PflMI    PpuMI    PshAI
     PsrI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbeI     BbsI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI