(Linear) (Six-base) MAPSORT of: KIEE6166.seq  Check: 9931  from: 1  to: 240



KIEE6166.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:27  ..

BmtI G_CTAG'C

Cuts at:      0     127     240 
   Size:        127     113
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      19     240 
   Size:         19     221
 
BsmI GAATG_Cn'

Cuts at:      0      47     240 
   Size:         47     193
 
BspHI T'CATG_A

Cuts at:      0      99     240 
   Size:         99     141
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     227     240 
   Size:        227      13
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     159     194     240 
   Size:        159      35      46

  Fragments arranged by size: 

                 159      46      35
 
EaeI y'GGCC_r

Cuts at:      0      11     240 
   Size:         11     229
 
MscI TGG'CCA

Cuts at:      0      13     240 
   Size:         13     227
 
MslI CAynn'nnrTG

Cuts at:      0      74     240 
   Size:         74     166
 
NheI G'CTAG_C

Cuts at:      0     123     240 
   Size:        123     117
 
SmlI C'TyrA_G

Cuts at:      0      34     240 
   Size:         34     206
 
StyI C'CwwG_G

Cuts at:      0       8     240 
   Size:          8     232
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     147     240 
   Size:        147      93
 

 Enzymes that do cut:

     BmtI    BpuEI     BsmI    BspHI    BstXI    CspCI     EaeI     MscI
     MslI     NheI     SmlI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI