(Linear) (Six-base) MAPSORT of: KIEE6165.seq  Check: 4077  from: 1  to: 441



KIEE6165.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:27  ..

AccI GT'mk_AC

Cuts at:      0     104     441 
   Size:        104     337
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     294     441 
   Size:        294     147
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     217     441 
   Size:        217     224
 
ApoI r'AATT_y

Cuts at:      0     364     441 
   Size:        364      77
 
BamHI G'GATC_C

Cuts at:      0     297     441 
   Size:        297     144
 
BanI G'GyrC_C

Cuts at:      0     208     441 
   Size:        208     233
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      31     441 
   Size:         31     410
 
Bme1580I G_kGCm'C

Cuts at:      0     253     441 
   Size:        253     188
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     441 
   Size:        229     212
 
BsaAI yAC'GTr

Cuts at:      0     294     441 
   Size:        294     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      34     441 
   Size:         34     407
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     274     441 
   Size:        259      15     167

  Fragments arranged by size: 

                 259     167      15
 
Bsp1286I G_dGCh'C

Cuts at:      0     253     441 
   Size:        253     188
 
BsrDI GCAATG_nn'

Cuts at:      0     330     441 
   Size:        330     111
 
BstYI r'GATC_y

Cuts at:      0     141     297     441 
   Size:        141     156     144

  Fragments arranged by size: 

                 156     144     141
 
Bsu36I CC'TnA_GG

Cuts at:      0      17     176     441 
   Size:         17     159     265

  Fragments arranged by size: 

                 265     159      17
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     294     441 
   Size:        294     147
 
EcoO109I rG'GnC_Cy

Cuts at:      0      81     441 
   Size:         81     360
 
EcoRI G'AATT_C

Cuts at:      0     364     441 
   Size:        364      77
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     133     165     441 
   Size:        133      32     276

  Fragments arranged by size: 

                 276     133      32
 
MspA1I CmG'CkG

Cuts at:      0     264     441 
   Size:        264     177
 
PasI CC'CwG_GG

Cuts at:      0      84     441 
   Size:         84     357
 
PmlI CAC'GTG

Cuts at:      0     294     441 
   Size:        294     147
 
PshAI GACnn'nnGTC

Cuts at:      0      31     441 
   Size:         31     410
 
PvuII CAG'CTG

Cuts at:      0     264     441 
   Size:        264     177
 
SmlI C'TyrA_G

Cuts at:      0     244     441 
   Size:        244     197
 
StyI C'CwwG_G

Cuts at:      0     109     441 
   Size:        109     332
 

 Enzymes that do cut:

     AccI     AcuI     AlfI     ApoI    BamHI     BanI     BbsI Bme1580I
    BpuEI    BsaAI    BseRI     BsgI Bsp1286I    BsrDI    BstYI   Bsu36I
  Eco57MI EcoO109I    EcoRI    Hin4I   MspA1I     PasI     PmlI    PshAI
    PvuII     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI