(Linear) (Six-base) MAPSORT of: KIEE6162.seq  Check: 297  from: 1  to: 516



KIEE6162.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:27  ..

AflIII A'CryG_T

Cuts at:      0     176     516 
   Size:        176     340
 
AvaI C'yCGr_G

Cuts at:      0     397     516 
   Size:        397     119
 
BanII G_rGCy'C

Cuts at:      0     328     382     516 
   Size:        328      54     134

  Fragments arranged by size: 

                 328     134      54
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     399     516 
   Size:        399     117
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     258     509     516 
   Size:        258     251       7

  Fragments arranged by size: 

                 258     251       7
 
Bpu10I CC'TnA_GC

Cuts at:      0     317     516 
   Size:        317     199
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     372     415     516 
   Size:        372      43     101

  Fragments arranged by size: 

                 372     101      43
 
BseYI C'CCAG_C

Cuts at:      0     472     516 
   Size:        472      44
 
BsiHKAI G_wGCw'C

Cuts at:      0     328     382     516 
   Size:        328      54     134

  Fragments arranged by size: 

                 328     134      54
 
BsmI GAATG_Cn'

Cuts at:      0     121     516 
   Size:        121     395
 
Bsp1286I G_dGCh'C

Cuts at:      0     328     382     516 
   Size:        328      54     134

  Fragments arranged by size: 

                 328     134      54
 
BtgI C'CryG_G

Cuts at:      0      74     516 
   Size:         74     442
 
EaeI y'GGCC_r

Cuts at:      0       2     516 
   Size:          2     514
 
EarI CTCTTCn'nnn_

Cuts at:      0     163     516 
   Size:        163     353
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     177     516 
   Size:        177     339
 
EcoICRI GAG'CTC

Cuts at:      0     326     380     516 
   Size:        326      54     136

  Fragments arranged by size: 

                 326     136      54
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     258     509     516 
   Size:        258     251       7

  Fragments arranged by size: 

                 258     251       7
 
EcoO109I rG'GnC_Cy

Cuts at:      0     451     497     516 
   Size:        451      46      19

  Fragments arranged by size: 

                 451      46      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     484     516 
   Size:        484      32
 
MfeI C'AATT_G

Cuts at:      0     505     516 
   Size:        505      11
 
MscI TGG'CCA

Cuts at:      0       4     516 
   Size:          4     512
 
MslI CAynn'nnrTG

Cuts at:      0      73     516 
   Size:         73     443
 
NspI r_CATG'y

Cuts at:      0     180     233     516 
   Size:        180      53     283

  Fragments arranged by size: 

                 283     180      53
 
PciI A'CATG_T

Cuts at:      0     176     516 
   Size:        176     340
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     425     457     516 
   Size:        425      32      59

  Fragments arranged by size: 

                 425      59      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     451     497     516 
   Size:        451      46      19

  Fragments arranged by size: 

                 451      46      19
 
SacI G_AGCT'C

Cuts at:      0     328     382     516 
   Size:        328      54     134

  Fragments arranged by size: 

                 328     134      54
 
SanDI GG'GwC_CC

Cuts at:      0     451     516 
   Size:        451      65
 
SapI GCTCTTCn'nnn_

Cuts at:      0     163     516 
   Size:        163     353
 
SfcI C'TryA_G

Cuts at:      0      33     516 
   Size:         33     483
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      91     123     516 
   Size:         91      32     393

  Fragments arranged by size: 

                 393      91      32
 

 Enzymes that do cut:

   AflIII     AvaI    BanII     BplI     BpmI   Bpu10I    BseRI    BseYI
  BsiHKAI     BsmI Bsp1286I     BtgI     EaeI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    Hin4I     MfeI     MscI     MslI     NspI     PciI
     PpiI    PpuMI     SacI    SanDI     SapI     SfcI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII    SacII     SalI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI