(Linear) (Six-base) MAPSORT of: KIEE6160.seq  Check: 3433  from: 1  to: 348



KIEE6160.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      84     348 
   Size:         84     264
 
ApoI r'AATT_y

Cuts at:      0     317     348 
   Size:        317      31
 
BanII G_rGCy'C

Cuts at:      0     224     348 
   Size:        224     124
 
BglII A'GATC_T

Cuts at:      0     294     348 
   Size:        294      54
 
BlpI GC'TnA_GC

Cuts at:      0      46     307     348 
   Size:         46     261      41

  Fragments arranged by size: 

                 261      46      41
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     348 
   Size:         47     301
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     348 
   Size:        208     140
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19      22     107     348 
   Size:         19       3      85     241

  Fragments arranged by size: 

                 241      85      19       3
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     262     348 
   Size:        262      86
 
Bsp1286I G_dGCh'C

Cuts at:      0     224     348 
   Size:        224     124
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      84     348 
   Size:         84     264
 
BstEII G'GTnAC_C

Cuts at:      0     157     348 
   Size:        157     191
 
BstYI r'GATC_y

Cuts at:      0     191     294     348 
   Size:        191     103      54

  Fragments arranged by size: 

                 191     103      54
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     348 
   Size:         47     301
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     205     252     348 
   Size:        205      47      96

  Fragments arranged by size: 

                 205      96      47
 
EcoO109I rG'GnC_Cy

Cuts at:      0     130     348 
   Size:        130     218
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     279     311     348 
   Size:        279      32      37

  Fragments arranged by size: 

                 279      37      32
 
NspI r_CATG'y

Cuts at:      0     306     348 
   Size:        306      42
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     289     348 
   Size:        289      59
 
PpuMI rG'GwC_Cy

Cuts at:      0     130     348 
   Size:        130     218
 
SanDI GG'GwC_CC

Cuts at:      0     130     348 
   Size:        130     218
 
SmlI C'TyrA_G

Cuts at:      0     187     348 
   Size:        187     161
 
SphI G_CATG'C

Cuts at:      0     306     348 
   Size:        306      42
 
StyI C'CwwG_G

Cuts at:      0     323     348 
   Size:        323      25
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     145     348 
   Size:        145     203
 

 Enzymes that do cut:

     AarI     ApoI    BanII    BglII     BlpI     BpmI    BpuEI    BseRI
    BsmBI Bsp1286I    BspMI   BstEII    BstYI  Eco57MI    EcoNI EcoO109I
    Hin4I     NspI    PflMI    PpuMI    SanDI     SmlI     SphI     StyI
    TaqII

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI