(Linear) (Six-base) MAPSORT of: KIEE6159.seq  Check: 6574  from: 1  to: 363



KIEE6159.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:26  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     144     156     363 
   Size:        144      12     207

  Fragments arranged by size: 

                 207     144      12
 
ApaI G_GGCC'C

Cuts at:      0      75     363 
   Size:         75     288
 
AvaI C'yCGr_G

Cuts at:      0      37     363 
   Size:         37     326
 
BanII G_rGCy'C

Cuts at:      0      46      75     110     363 
   Size:         46      29      35     253

  Fragments arranged by size: 

                 253      46      35      29
 
BglI GCCn_nnn'nGGC

Cuts at:      0     306     363 
   Size:        306      57
 
BlpI GC'TnA_GC

Cuts at:      0     109     211     363 
   Size:        109     102     152

  Fragments arranged by size: 

                 152     109     102
 
Bme1580I G_kGCm'C

Cuts at:      0      75     363 
   Size:         75     288
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     363 
   Size:        121     242
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     293     363 
   Size:        293      70
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     273     303     363 
   Size:        273      30      60

  Fragments arranged by size: 

                 273      60      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25     363 
   Size:         25     338
 
BsiHKAI G_wGCw'C

Cuts at:      0     110     363 
   Size:        110     253
 
Bsp1286I G_dGCh'C

Cuts at:      0      46      75     110     363 
   Size:         46      29      35     253

  Fragments arranged by size: 

                 253      46      35      29
 
BssHII G'CGCG_C

Cuts at:      0      52     128     363 
   Size:         52      76     235

  Fragments arranged by size: 

                 235      76      52
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      36     363 
   Size:         36     327
 
EcoICRI GAG'CTC

Cuts at:      0     108     363 
   Size:        108     255
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     363 
   Size:        121     242
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     121     204     363 
   Size:        121      83     159

  Fragments arranged by size: 

                 159     121      83
 
EcoO109I rG'GnC_Cy

Cuts at:      0      71     153     281     363 
   Size:         71      82     128      82

  Fragments arranged by size: 

                 128      82      82      71
 
HaeII r_GCGC'y

Cuts at:      0     335     363 
   Size:        335      28
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     273     305     363 
   Size:        273      32      58

  Fragments arranged by size: 

                 273      58      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     266     363 
   Size:        266      97
 
MspA1I CmG'CkG

Cuts at:      0     141     328     363 
   Size:        141     187      35

  Fragments arranged by size: 

                 187     141      35
 
NspI r_CATG'y

Cuts at:      0     249     363 
   Size:        249     114
 
PasI CC'CwG_GG

Cuts at:      0      68     304     363 
   Size:         68     236      59

  Fragments arranged by size: 

                 236      68      59
 
PpuMI rG'GwC_Cy

Cuts at:      0     281     363 
   Size:        281      82
 
PspOMI G'GGCC_C

Cuts at:      0      71     363 
   Size:         71     292
 
PstI C_TGCA'G

Cuts at:      0     260     342     363 
   Size:        260      82      21

  Fragments arranged by size: 

                 260      82      21
 
PvuII CAG'CTG

Cuts at:      0     328     363 
   Size:        328      35
 
SacI G_AGCT'C

Cuts at:      0     110     363 
   Size:        110     253
 
SbfI CC_TGCA'GG

Cuts at:      0     260     363 
   Size:        260     103
 
SexAI A'CCwGG_T

Cuts at:      0     283     363 
   Size:        283      80
 
SfcI C'TryA_G

Cuts at:      0     148     256     338     363 
   Size:        148     108      82      25

  Fragments arranged by size: 

                 148     108      82      25
 
StuI AGG'CCT

Cuts at:      0     262     363 
   Size:        262     101
 
Tth111I GACn'n_nGTC

Cuts at:      0     285     363 
   Size:        285      78
 

 Enzymes that do cut:

    AlwNI     ApaI     AvaI    BanII     BglI     BlpI Bme1580I     BpmI
     BsaI    BsaXI    BseRI  BsiHKAI Bsp1286I   BssHII     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     MmeI   MspA1I     NspI
     PasI    PpuMI   PspOMI     PstI    PvuII     SacI     SbfI    SexAI
     SfcI     StuI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
    TaqII     TatI     TstI     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI