(Linear) (Six-base) MAPSORT of: KIEE6158.seq  Check: 8764  from: 1  to: 360



KIEE6158.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:26  ..

BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     360 
   Size:        158     202
 
BsaBI GATnn'nnATC

Cuts at:      0     265     360 
   Size:        265      95
 
BsiHKAI G_wGCw'C

Cuts at:      0     218     360 
   Size:        218     142
 
Bsp1286I G_dGCh'C

Cuts at:      0     218     360 
   Size:        218     142
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     306     360 
   Size:        306      54
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     136     360 
   Size:        136     224
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     360 
   Size:        158     202
 
EcoO109I rG'GnC_Cy

Cuts at:      0      78     360 
   Size:         78     282
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     131     163     360 
   Size:        131      32     197

  Fragments arranged by size: 

                 197     131      32
 

 Enzymes that do cut:

     BpmI    BsaBI  BsiHKAI Bsp1286I   BstAPI    BstXI  Eco57MI EcoO109I
    Hin4I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI