(Linear) (Six-base) MAPSORT of: KIEE6153.seq  Check: 6722  from: 1  to: 423



KIEE6153.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:26  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      49     423 
   Size:         49     374
 
ApoI r'AATT_y

Cuts at:      0     159     186     244     423 
   Size:        159      27      58     179

  Fragments arranged by size: 

                 179     159      58      27
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     265     423 
   Size:        265     158
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     423 
   Size:        277     146
 
BsmI GAATG_Cn'

Cuts at:      0       9     423 
   Size:          9     414
 
BspHI T'CATG_A

Cuts at:      0      81     423 
   Size:         81     342
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     252     423 
   Size:        252     171
 
BstYI r'GATC_y

Cuts at:      0      19     423 
   Size:         19     404
 
BtsI GCAGTG_nn'

Cuts at:      0     260     423 
   Size:        260     163
 
EcoRI G'AATT_C

Cuts at:      0     244     423 
   Size:        244     179
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      86     423 
   Size:         86     337
 

 Enzymes that do cut:

    AlwNI     ApoI     BbsI     BsgI     BsmI    BspHI    BspMI    BstYI
     BtsI    EcoRI     FalI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI