(Linear) (Six-base) MAPSORT of: KIEE6151.seq  Check: 2204  from: 1  to: 291



KIEE6151.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:26  ..

AfeI AGC'GCT

Cuts at:      0      42     291 
   Size:         42     249
 
AvaI C'yCGr_G

Cuts at:      0      26     150     169     291 
   Size:         26     124      19     122

  Fragments arranged by size: 

                 124     122      26      19
 
BanII G_rGCy'C

Cuts at:      0      75     291 
   Size:         75     216
 
BglI GCCn_nnn'nGGC

Cuts at:      0      15     291 
   Size:         15     276
 
BsaAI yAC'GTr

Cuts at:      0     258     291 
   Size:        258      33
 
BsaHI Gr'CG_yC

Cuts at:      0      84     291 
   Size:         84     207
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      77     291 
   Size:         77     214
 
BseYI C'CCAG_C

Cuts at:      0     112     291 
   Size:        112     179
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     111     291 
   Size:        111     180
 
BsiHKAI G_wGCw'C

Cuts at:      0      75     291 
   Size:         75     216
 
Bsp1286I G_dGCh'C

Cuts at:      0      75     291 
   Size:         75     216
 
BtgI C'CryG_G

Cuts at:      0      57     272     291 
   Size:         57     215      19

  Fragments arranged by size: 

                 215      57      19
 
EaeI y'GGCC_r

Cuts at:      0      35     291 
   Size:         35     256
 
EcoICRI GAG'CTC

Cuts at:      0      73     291 
   Size:         73     218
 
EcoO109I rG'GnC_Cy

Cuts at:      0      17     291 
   Size:         17     274
 
HaeII r_GCGC'y

Cuts at:      0      44     109     291 
   Size:         44      65     182

  Fragments arranged by size: 

                 182      65      44
 
MscI TGG'CCA

Cuts at:      0      37     291 
   Size:         37     254
 
PasI CC'CwG_GG

Cuts at:      0     218     291 
   Size:        218      73
 
PmlI CAC'GTG

Cuts at:      0     258     291 
   Size:        258      33
 
SacI G_AGCT'C

Cuts at:      0      75     291 
   Size:         75     216
 
SmaI CCC'GGG

Cuts at:      0      28     291 
   Size:         28     263
 
XmaI C'CCGG_G

Cuts at:      0      26     291 
   Size:         26     265
 

 Enzymes that do cut:

     AfeI     AvaI    BanII     BglI    BsaAI    BsaHI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I     BtgI     EaeI  EcoICRI EcoO109I    HaeII
     MscI     PasI     PmlI     SacI     SmaI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI