(Linear) (Six-base) MAPSORT of: KIEE6150.seq  Check: 3268  from: 1  to: 411



KIEE6150.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:26  ..

AleI CACnn'nnGTG

Cuts at:      0     172     411 
   Size:        172     239
 
ApoI r'AATT_y

Cuts at:      0      42     395     411 
   Size:         42     353      16

  Fragments arranged by size: 

                 353      42      16
 
BanI G'GyrC_C

Cuts at:      0      16     370     411 
   Size:         16     354      41

  Fragments arranged by size: 

                 354      41      16
 
BbeI G_GCGC'C

Cuts at:      0     374     411 
   Size:        374      37
 
BbvCI CC'TCA_GC

Cuts at:      0      85     411 
   Size:         85     326
 
BglI GCCn_nnn'nGGC

Cuts at:      0     103     411 
   Size:        103     308
 
BmgBI CAC'GTC

Cuts at:      0     381     411 
   Size:        381      30
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      76     411 
   Size:         76     335
 
Bpu10I CC'TnA_GC

Cuts at:      0      85     411 
   Size:         85     326
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     411 
   Size:         43     368
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     217     411 
   Size:        217     194
 
BsaAI yAC'GTr

Cuts at:      0     123     411 
   Size:        123     288
 
BsaHI Gr'CG_yC

Cuts at:      0     371     411 
   Size:        371      40
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     232     235     411 
   Size:        232       3     176

  Fragments arranged by size: 

                 232     176       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     264     270     372     411 
   Size:        264       6     102      39

  Fragments arranged by size: 

                 264     102      39       6
 
BsrFI r'CCGG_y

Cuts at:      0      13     411 
   Size:         13     398
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     339     411 
   Size:        339      72
 
BstYI r'GATC_y

Cuts at:      0     128     278     411 
   Size:        128     150     133

  Fragments arranged by size: 

                 150     133     128
 
BtgI C'CryG_G

Cuts at:      0     170     227     411 
   Size:        170      57     184

  Fragments arranged by size: 

                 184     170      57
 
BtsI GCAGTG_nn'

Cuts at:      0     282     411 
   Size:        282     129
 
DraIII CAC_nnn'GTG

Cuts at:      0      70     411 
   Size:         70     341
 
EaeI y'GGCC_r

Cuts at:      0      95     341     411 
   Size:         95     246      70

  Fragments arranged by size: 

                 246      95      70
 
EarI CTCTTCn'nnn_

Cuts at:      0     302     411 
   Size:        302     109
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     121     411 
   Size:        121     290
 
FspI TGC'GCA

Cuts at:      0      31     178     411 
   Size:         31     147     233

  Fragments arranged by size: 

                 233     147      31
 
FspAI rTGC'GCAy

Cuts at:      0     178     411 
   Size:        178     233
 
HaeII r_GCGC'y

Cuts at:      0     374     411 
   Size:        374      37
 
KasI G'GCGC_C

Cuts at:      0     370     411 
   Size:        370      41
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     183     411 
   Size:        183     228
 
MscI TGG'CCA

Cuts at:      0     343     411 
   Size:        343      68
 
MslI CAynn'nnrTG

Cuts at:      0     172     411 
   Size:        172     239
 
NaeI GCC'GGC

Cuts at:      0      15     411 
   Size:         15     396
 
NarI GG'CG_CC

Cuts at:      0     371     411 
   Size:        371      40
 
NgoMIV G'CCGG_C

Cuts at:      0      13     411 
   Size:         13     398
 
PmlI CAC'GTG

Cuts at:      0     123     411 
   Size:        123     288
 
SfoI GGC'GCC

Cuts at:      0     372     411 
   Size:        372      39
 
SmlI C'TyrA_G

Cuts at:      0      58     411 
   Size:         58     353
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      58     411 
   Size:         58     353
 
Tth111I GACn'n_nGTC

Cuts at:      0     382     411 
   Size:        382      29
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     120     411 
   Size:        120     291
 

 Enzymes that do cut:

     AleI     ApoI     BanI     BbeI    BbvCI     BglI    BmgBI     BmrI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BseRI     BsgI    BsrFI
    BstXI    BstYI     BtgI     BtsI   DraIII     EaeI     EarI     EciI
     FspI    FspAI    HaeII     KasI     MmeI     MscI     MslI     NaeI
     NarI   NgoMIV     PmlI     SfoI     SmlI    TaqII  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbsI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
     BmtI     BplI     BpmI    BsaBI    BsaWI    BsaXI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI   MspA1I
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI     XbaI
     XhoI     XmaI     XmnI     ZraI