(Linear) (Six-base) MAPSORT of: KIEE6149.seq  Check: 4454  from: 1  to: 435



KIEE6149.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     298     435 
   Size:        298     137
 
AseI AT'TA_AT

Cuts at:      0     371     435 
   Size:        371      64
 
AvaI C'yCGr_G

Cuts at:      0      59     156     435 
   Size:         59      97     279

  Fragments arranged by size: 

                 279      97      59
 
BanII G_rGCy'C

Cuts at:      0      38     435 
   Size:         38     397
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     244     435 
   Size:        244     191
 
BbvCI CC'TCA_GC

Cuts at:      0     277     435 
   Size:        277     158
 
Bme1580I G_kGCm'C

Cuts at:      0     197     262     435 
   Size:        197      65     173

  Fragments arranged by size: 

                 197     173      65
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     249     435 
   Size:        249     186
 
Bpu10I CC'TnA_GC

Cuts at:      0     277     282     435 
   Size:        277       5     153

  Fragments arranged by size: 

                 277     153       5
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     244     435 
   Size:        244     191
 
BseYI C'CCAG_C

Cuts at:      0     109     435 
   Size:        109     326
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     435 
   Size:        136     299
 
BsmI GAATG_Cn'

Cuts at:      0     353     435 
   Size:        353      82
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     197     262     435 
   Size:         38     159      65     173

  Fragments arranged by size: 

                 173     159      65      38
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      37     435 
   Size:         37     398
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     298     435 
   Size:        298     137
 
EcoO109I rG'GnC_Cy

Cuts at:      0     286     435 
   Size:        286     149
 
HaeII r_GCGC'y

Cuts at:      0     116     435 
   Size:        116     319
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      55      87     435 
   Size:         55      32     348

  Fragments arranged by size: 

                 348      55      32
 
HincII GTy'rAC

Cuts at:      0     432     435 
   Size:        432       3
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     414     435 
   Size:        414      21
 
MspA1I CmG'CkG

Cuts at:      0     131     435 
   Size:        131     304
 
NspI r_CATG'y

Cuts at:      0     165     435 
   Size:        165     270
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     396     435 
   Size:        396      39
 
PshAI GACnn'nnGTC

Cuts at:      0     404     435 
   Size:        404      31
 
SfcI C'TryA_G

Cuts at:      0     203     435 
   Size:        203     232
 
SmaI CCC'GGG

Cuts at:      0      61     435 
   Size:         61     374
 
SphI G_CATG'C

Cuts at:      0     165     435 
   Size:        165     270
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     304     435 
   Size:        304     131
 
XmaI C'CCGG_G

Cuts at:      0      59     435 
   Size:         59     376
 

 Enzymes that do cut:

     AcuI     AseI     AvaI    BanII     BbsI    BbvCI Bme1580I     BmrI
   Bpu10I     BsaI    BseYI     BsgI     BsmI Bsp1286I     EciI  Eco57MI
 EcoO109I    HaeII    Hin4I   HincII     MmeI   MspA1I     NspI    PflMI
    PshAI     SfcI     SmaI     SphI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI