(Linear) (Six-base) MAPSORT of: KIEE6148.seq  Check: 5072  from: 1  to: 306



KIEE6148.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     153     306 
   Size:        153     153
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     286     306 
   Size:        119     167      20

  Fragments arranged by size: 

                 167     119      20
 
AflII C'TTAA_G

Cuts at:      0      10     306 
   Size:         10     296
 
AvaI C'yCGr_G

Cuts at:      0     282     306 
   Size:        282      24
 
BanII G_rGCy'C

Cuts at:      0     179     281     306 
   Size:        179     102      25

  Fragments arranged by size: 

                 179     102      25
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      69     110     306 
   Size:         69      41     196

  Fragments arranged by size: 

                 196      69      41
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     190     224     306 
   Size:        190      34      82

  Fragments arranged by size: 

                 190      82      34
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     291     306 
   Size:        291      15
 
BsiHKAI G_wGCw'C

Cuts at:      0     281     306 
   Size:        281      25
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      99     306 
   Size:         99     207
 
Bsp1286I G_dGCh'C

Cuts at:      0     179     281     306 
   Size:        179     102      25

  Fragments arranged by size: 

                 179     102      25
 
BspHI T'CATG_A

Cuts at:      0     273     306 
   Size:        273      33
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      63     153     306 
   Size:         63      90     153

  Fragments arranged by size: 

                 153      90      63
 
BstYI r'GATC_y

Cuts at:      0      24     306 
   Size:         24     282
 
EaeI y'GGCC_r

Cuts at:      0     230     245     306 
   Size:        230      15      61

  Fragments arranged by size: 

                 230      61      15
 
EarI CTCTTCn'nnn_

Cuts at:      0      11     306 
   Size:         11     295
 
EcoICRI GAG'CTC

Cuts at:      0     279     306 
   Size:        279      27
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     286     306 
   Size:        119     167      20

  Fragments arranged by size: 

                 167     119      20
 
MscI TGG'CCA

Cuts at:      0     232     247     306 
   Size:        232      15      59

  Fragments arranged by size: 

                 232      59      15
 
PstI C_TGCA'G

Cuts at:      0     203     306 
   Size:        203     103
 
SacI G_AGCT'C

Cuts at:      0     281     306 
   Size:        281      25
 
SapI GCTCTTCn'nnn_

Cuts at:      0      11     306 
   Size:         11     295
 
SfcI C'TryA_G

Cuts at:      0     199     306 
   Size:        199     107
 
SmlI C'TyrA_G

Cuts at:      0      10     306 
   Size:         10     296
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     305     306 
   Size:        305       1
 
TatI w'GTAC_w

Cuts at:      0     251     306 
   Size:        251      55
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     240     306 
   Size:        240      66
 
XmnI GAAnn'nnTTC

Cuts at:      0      49     306 
   Size:         49     257
 

 Enzymes that do cut:

     AarI     AcuI    AflII     AvaI    BanII     BbsI     BcgI    BseRI
  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BstYI     EaeI     EarI
  EcoICRI  Eco57MI     MscI     PstI     SacI     SapI     SfcI     SmlI
    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI