(Linear) (Six-base) MAPSORT of: KIEE6145.seq  Check: 5804  from: 1  to: 270



KIEE6145.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     270 
   Size:        203      67
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     173     270 
   Size:        173      97
 
BanII G_rGCy'C

Cuts at:      0     231     270 
   Size:        231      39
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     126     160     270 
   Size:        126      34     110

  Fragments arranged by size: 

                 126     110      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63     243     270 
   Size:         63     180      27

  Fragments arranged by size: 

                 180      63      27
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     270 
   Size:        220      50
 
BsaBI GATnn'nnATC

Cuts at:      0      52     270 
   Size:         52     218
 
BsiHKAI G_wGCw'C

Cuts at:      0     231     270 
   Size:        231      39
 
Bsp1286I G_dGCh'C

Cuts at:      0     231     270 
   Size:        231      39
 
BsrDI GCAATG_nn'

Cuts at:      0     233     270 
   Size:        233      37
 
BstBI TT'CG_AA

Cuts at:      0     254     270 
   Size:        254      16
 
BstYI r'GATC_y

Cuts at:      0      66     270 
   Size:         66     204
 
EcoICRI GAG'CTC

Cuts at:      0     229     270 
   Size:        229      41
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63     203     243     270 
   Size:         63     140      40      27

  Fragments arranged by size: 

                 140      63      40      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0       6     270 
   Size:          6     264
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      53     270 
   Size:         53     217
 
PpuMI rG'GwC_Cy

Cuts at:      0       6     270 
   Size:          6     264
 
PstI C_TGCA'G

Cuts at:      0     162     270 
   Size:        162     108
 
SacI G_AGCT'C

Cuts at:      0     231     270 
   Size:        231      39
 
SanDI GG'GwC_CC

Cuts at:      0       6     270 
   Size:          6     264
 
SfcI C'TryA_G

Cuts at:      0     158     270 
   Size:        158     112
 
SmlI C'TyrA_G

Cuts at:      0     199     270 
   Size:        199      71
 
StuI AGG'CCT

Cuts at:      0      86     270 
   Size:         86     184
 
TatI w'GTAC_w

Cuts at:      0      23     203     270 
   Size:         23     180      67

  Fragments arranged by size: 

                 180      67      23
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      60     270 
   Size:         60     210
 

 Enzymes that do cut:

     AcuI     AlfI    BanII     BcgI     BpmI    BpuEI    BsaBI  BsiHKAI
 Bsp1286I    BsrDI    BstBI    BstYI  EcoICRI  Eco57MI EcoO109I     MmeI
    PpuMI     PstI     SacI    SanDI     SfcI     SmlI     StuI     TatI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI