(Linear) (Six-base) MAPSORT of: KIEE6142.seq  Check: 4946  from: 1  to: 327



KIEE6142.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     327 
   Size:        196     131
 
AvaI C'yCGr_G

Cuts at:      0     142     205     327 
   Size:        142      63     122

  Fragments arranged by size: 

                 142     122      63
 
BanI G'GyrC_C

Cuts at:      0      79     327 
   Size:         79     248
 
BanII G_rGCy'C

Cuts at:      0     100     327 
   Size:        100     227
 
BbeI G_GCGC'C

Cuts at:      0      83     327 
   Size:         83     244
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     121     327 
   Size:        121     206
 
BlpI GC'TnA_GC

Cuts at:      0      99     327 
   Size:         99     228
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     195     327 
   Size:        195     132
 
BsaHI Gr'CG_yC

Cuts at:      0      80     327 
   Size:         80     247
 
BseYI C'CCAG_C

Cuts at:      0      36      57     327 
   Size:         36      21     270

  Fragments arranged by size: 

                 270      36      21
 
BsiHKAI G_wGCw'C

Cuts at:      0     197     327 
   Size:        197     130
 
Bsp1286I G_dGCh'C

Cuts at:      0     100     197     327 
   Size:        100      97     130

  Fragments arranged by size: 

                 130     100      97
 
BssSI C'ACGA_G

Cuts at:      0     196     327 
   Size:        196     131
 
BstEII G'GTnAC_C

Cuts at:      0     265     327 
   Size:        265      62
 
BtgI C'CryG_G

Cuts at:      0      84     186     327 
   Size:         84     102     141

  Fragments arranged by size: 

                 141     102      84
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     198     327 
   Size:        198     129
 
EarI CTCTTCn'nnn_

Cuts at:      0      86     327 
   Size:         86     241
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     327 
   Size:        196     131
 
HaeII r_GCGC'y

Cuts at:      0      83     327 
   Size:         83     244
 
KasI G'GCGC_C

Cuts at:      0      79     327 
   Size:         79     248
 
MspA1I CmG'CkG

Cuts at:      0      57     327 
   Size:         57     270
 
NarI GG'CG_CC

Cuts at:      0      80     327 
   Size:         80     247
 
NcoI C'CATG_G

Cuts at:      0      84     327 
   Size:         84     243
 
NspI r_CATG'y

Cuts at:      0     111     162     327 
   Size:        111      51     165

  Fragments arranged by size: 

                 165     111      51
 
PasI CC'CwG_GG

Cuts at:      0     254     327 
   Size:        254      73
 
PvuII CAG'CTG

Cuts at:      0      57     327 
   Size:         57     270
 
SfoI GGC'GCC

Cuts at:      0      81     327 
   Size:         81     246
 
SmaI CCC'GGG

Cuts at:      0     144     327 
   Size:        144     183
 
SphI G_CATG'C

Cuts at:      0     162     327 
   Size:        162     165
 
StyI C'CwwG_G

Cuts at:      0      84     327 
   Size:         84     243
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      32     327 
   Size:         32     295
 
XmaI C'CCGG_G

Cuts at:      0     142     327 
   Size:        142     185
 

 Enzymes that do cut:

     AcuI     AvaI     BanI    BanII     BbeI     BbsI     BlpI     BsaI
    BsaHI    BseYI  BsiHKAI Bsp1286I    BssSI   BstEII     BtgI    BtgZI
     EarI  Eco57MI    HaeII     KasI   MspA1I     NarI     NcoI     NspI
     PasI    PvuII     SfoI     SmaI     SphI     StyI    TaqII     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmnI     ZraI