(Linear) (Six-base) MAPSORT of: KIEE6139.seq  Check: 5628  from: 1  to: 285



KIEE6139.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:25  ..

AatII G_ACGT'C

Cuts at:      0     266     285 
   Size:        266      19
 
ApaI G_GGCC'C

Cuts at:      0      43     285 
   Size:         43     242
 
AvaI C'yCGr_G

Cuts at:      0      11     178     231     285 
   Size:         11     167      53      54

  Fragments arranged by size: 

                 167      54      53      11
 
BanI G'GyrC_C

Cuts at:      0       7     285 
   Size:          7     278
 
BanII G_rGCy'C

Cuts at:      0      43     260     285 
   Size:         43     217      25

  Fragments arranged by size: 

                 217      43      25
 
BbeI G_GCGC'C

Cuts at:      0      11     285 
   Size:         11     274
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     162     285 
   Size:        162     123
 
Bme1580I G_kGCm'C

Cuts at:      0      43     285 
   Size:         43     242
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      46     285 
   Size:         46     239
 
BsaAI yAC'GTr

Cuts at:      0      57      90     285 
   Size:         57      33     195

  Fragments arranged by size: 

                 195      57      33
 
BsaHI Gr'CG_yC

Cuts at:      0       8     263     285 
   Size:          8     255      22

  Fragments arranged by size: 

                 255      22       8
 
BsiHKAI G_wGCw'C

Cuts at:      0     218     260     285 
   Size:        218      42      25

  Fragments arranged by size: 

                 218      42      25
 
Bsp1286I G_dGCh'C

Cuts at:      0      43     218     260     285 
   Size:         43     175      42      25

  Fragments arranged by size: 

                 175      43      42      25
 
BssSI C'ACGA_G

Cuts at:      0     199     285 
   Size:        199      86
 
EcoICRI GAG'CTC

Cuts at:      0     258     285 
   Size:        258      27
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     178     285 
   Size:        178     107
 
EcoO109I rG'GnC_Cy

Cuts at:      0      40     285 
   Size:         40     245
 
HaeII r_GCGC'y

Cuts at:      0      11     199     285 
   Size:         11     188      86

  Fragments arranged by size: 

                 188      86      11
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     214     235     246     267     285 
   Size:        214      21      11      21      18

  Fragments arranged by size: 

                 214      21      21      18      11
 
KasI G'GCGC_C

Cuts at:      0       7     285 
   Size:          7     278
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     285 
   Size:        245      40
 
MspA1I CmG'CkG

Cuts at:      0      69     285 
   Size:         69     216
 
NarI GG'CG_CC

Cuts at:      0       8     285 
   Size:          8     277
 
PspOMI G'GGCC_C

Cuts at:      0      39     285 
   Size:         39     246
 
SacI G_AGCT'C

Cuts at:      0     260     285 
   Size:        260      25
 
SfoI GGC'GCC

Cuts at:      0       9     285 
   Size:          9     276
 
SmaI CCC'GGG

Cuts at:      0      13     285 
   Size:         13     272
 
TatI w'GTAC_w

Cuts at:      0     122     143     285 
   Size:        122      21     142

  Fragments arranged by size: 

                 142     122      21
 
XmaI C'CCGG_G

Cuts at:      0      11     285 
   Size:         11     274
 
ZraI GAC'GTC

Cuts at:      0     264     285 
   Size:        264      21
 

 Enzymes that do cut:

    AatII     ApaI     AvaI     BanI    BanII     BbeI     BbsI Bme1580I
     BmrI    BsaAI    BsaHI  BsiHKAI Bsp1286I    BssSI  EcoICRI    EcoNI
 EcoO109I    HaeII    Hin4I     KasI     MmeI   MspA1I     NarI   PspOMI
     SacI     SfoI     SmaI     TatI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MscI
     MslI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI