(Linear) (Six-base) MAPSORT of: KIEE6138.seq  Check: 3296  from: 1  to: 405



KIEE6138.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:25  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     337     405 
   Size:        334       3      68

  Fragments arranged by size: 

                 334      68       3
 
ApoI r'AATT_y

Cuts at:      0     179     378     405 
   Size:        179     199      27

  Fragments arranged by size: 

                 199     179      27
 
AvaI C'yCGr_G

Cuts at:      0      38     392     405 
   Size:         38     354      13

  Fragments arranged by size: 

                 354      38      13
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     187     405 
   Size:        187     218
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     267     405 
   Size:        267     138
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     121     238     241     405 
   Size:        121     117       3     164

  Fragments arranged by size: 

                 164     121     117       3
 
BseYI C'CCAG_C

Cuts at:      0     124     405 
   Size:        124     281
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     142     405 
   Size:        142     263
 
BstYI r'GATC_y

Cuts at:      0     294     405 
   Size:        294     111
 
Bsu36I CC'TnA_GG

Cuts at:      0     257     405 
   Size:        257     148
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     337     405 
   Size:        334       3      68

  Fragments arranged by size: 

                 334      68       3
 
EcoO109I rG'GnC_Cy

Cuts at:      0     397     405 
   Size:        397       8
 
EcoRI G'AATT_C

Cuts at:      0     179     405 
   Size:        179     226
 
HindIII A'AGCT_T

Cuts at:      0     319     405 
   Size:        319      86
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     368     405 
   Size:        368      37
 
PpuMI rG'GwC_Cy

Cuts at:      0     397     405 
   Size:        397       8
 
PstI C_TGCA'G

Cuts at:      0     221     405 
   Size:        221     184
 
ScaI AGT'ACT

Cuts at:      0      58     405 
   Size:         58     347
 
SfcI C'TryA_G

Cuts at:      0     217     365     405 
   Size:        217     148      40

  Fragments arranged by size: 

                 217     148      40
 
SmaI CCC'GGG

Cuts at:      0      40     405 
   Size:         40     365
 
StyI C'CwwG_G

Cuts at:      0     269     405 
   Size:        269     136
 
TatI w'GTAC_w

Cuts at:      0      56     405 
   Size:         56     349
 
XmaI C'CCGG_G

Cuts at:      0      38     405 
   Size:         38     367
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI     BmrI     BsaI    BseRI    BseYI    BspMI
    BstYI   Bsu36I  Eco57MI EcoO109I    EcoRI  HindIII     MmeI    PpuMI
     PstI     ScaI     SfcI     SmaI     StyI     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI