(Linear) (Six-base) MAPSORT of: KIEE6136.seq  Check: 2604  from: 1  to: 309



KIEE6136.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:25  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     270     309 
   Size:        270      39
 
BamHI G'GATC_C

Cuts at:      0     170     309 
   Size:        170     139
 
BanII G_rGCy'C

Cuts at:      0     203     309 
   Size:        203     106
 
BbvCI CC'TCA_GC

Cuts at:      0     137     309 
   Size:        137     172
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     253     309 
   Size:        253      56
 
BglI GCCn_nnn'nGGC

Cuts at:      0      28     309 
   Size:         28     281
 
Bpu10I CC'TnA_GC

Cuts at:      0      36     137     309 
   Size:         36     101     172

  Fragments arranged by size: 

                 172     101      36
 
BsiHKAI G_wGCw'C

Cuts at:      0     203     309 
   Size:        203     106
 
Bsp1286I G_dGCh'C

Cuts at:      0     203     309 
   Size:        203     106
 
BssSI C'ACGA_G

Cuts at:      0     196     309 
   Size:        196     113
 
BstYI r'GATC_y

Cuts at:      0     170     309 
   Size:        170     139
 
BtgI C'CryG_G

Cuts at:      0     248     309 
   Size:        248      61
 
EaeI y'GGCC_r

Cuts at:      0      29     309 
   Size:         29     280
 
EcoICRI GAG'CTC

Cuts at:      0     201     309 
   Size:        201     108
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     270     309 
   Size:        270      39
 
HaeII r_GCGC'y

Cuts at:      0     297     309 
   Size:        297      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     143     175     309 
   Size:        143      32     134

  Fragments arranged by size: 

                 143     134      32
 
NcoI C'CATG_G

Cuts at:      0     248     309 
   Size:        248      61
 
SacI G_AGCT'C

Cuts at:      0     203     309 
   Size:        203     106
 
SfcI C'TryA_G

Cuts at:      0     225     309 
   Size:        225      84
 
StyI C'CwwG_G

Cuts at:      0     248     309 
   Size:        248      61
 

 Enzymes that do cut:

     AcuI    BamHI    BanII    BbvCI    BciVI     BglI   Bpu10I  BsiHKAI
 Bsp1286I    BssSI    BstYI     BtgI     EaeI  EcoICRI  Eco57MI    HaeII
    Hin4I     NcoI     SacI     SfcI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI
     BbeI     BbsI     BcgI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI