(Linear) (Six-base) MAPSORT of: KIEE6135.seq  Check: 7876  from: 1  to: 315



KIEE6135.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:25  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     315 
   Size:        202     113
 
ApaI G_GGCC'C

Cuts at:      0     207     315 
   Size:        207     108
 
ApoI r'AATT_y

Cuts at:      0     211     315 
   Size:        211     104
 
AscI GG'CGCG_CC

Cuts at:      0     165     315 
   Size:        165     150
 
BanII G_rGCy'C

Cuts at:      0     207     315 
   Size:        207     108
 
BlpI GC'TnA_GC

Cuts at:      0     268     315 
   Size:        268      47
 
Bme1580I G_kGCm'C

Cuts at:      0     207     315 
   Size:        207     108
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     192     315 
   Size:        192     123
 
BsaHI Gr'CG_yC

Cuts at:      0      84      93     315 
   Size:         84       9     222

  Fragments arranged by size: 

                 222      84       9
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     277     315 
   Size:        277      38
 
BsiWI C'GTAC_G

Cuts at:      0     155     315 
   Size:        155     160
 
Bsp1286I G_dGCh'C

Cuts at:      0     207     315 
   Size:        207     108
 
BsrBI CCG'CTC

Cuts at:      0     162     315 
   Size:        162     153
 
BssHII G'CGCG_C

Cuts at:      0     165     315 
   Size:        165     150
 
BtgI C'CryG_G

Cuts at:      0      23      87     170     315 
   Size:         23      64      83     145

  Fragments arranged by size: 

                 145      83      64      23
 
DraIII CAC_nnn'GTG

Cuts at:      0      48     315 
   Size:         48     267
 
EaeI y'GGCC_r

Cuts at:      0      20     281     315 
   Size:         20     261      34

  Fragments arranged by size: 

                 261      34      20
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     315 
   Size:        202     113
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      34     315 
   Size:         34     281
 
EcoO109I rG'GnC_Cy

Cuts at:      0     204     315 
   Size:        204     111
 
MspA1I CmG'CkG

Cuts at:      0      89     315 
   Size:         89     226
 
NcoI C'CATG_G

Cuts at:      0     170     315 
   Size:        170     145
 
PspOMI G'GGCC_C

Cuts at:      0     203     315 
   Size:        203     112
 
SacII CC_GC'GG

Cuts at:      0      90     315 
   Size:         90     225
 
StyI C'CwwG_G

Cuts at:      0     170     191     315 
   Size:        170      21     124

  Fragments arranged by size: 

                 170     124      21
 

 Enzymes that do cut:

     AcuI     ApaI     ApoI     AscI    BanII     BlpI Bme1580I     BsaI
    BsaHI    BseRI    BsiWI Bsp1286I    BsrBI   BssHII     BtgI   DraIII
     EaeI  Eco57MI    EcoNI EcoO109I   MspA1I     NcoI   PspOMI    SacII
     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EagI     EarI     EciI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI