(Linear) (Six-base) MAPSORT of: KIEE6134.seq  Check: 5008  from: 1  to: 429



KIEE6134.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:25  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     162     429 
   Size:        162     267
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      17      50     429 
   Size:         17      33     379

  Fragments arranged by size: 

                 379      33      17
 
BanII G_rGCy'C

Cuts at:      0     385     429 
   Size:        385      44
 
BfrBI ATG'CAT

Cuts at:      0       9     429 
   Size:          9     420
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      72     429 
   Size:         72     357
 
BsiHKAI G_wGCw'C

Cuts at:      0      98     429 
   Size:         98     331
 
Bsp1286I G_dGCh'C

Cuts at:      0      98     385     429 
   Size:         98     287      44

  Fragments arranged by size: 

                 287      98      44
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     179     429 
   Size:        179     250
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      19     183     429 
   Size:         19     164     246

  Fragments arranged by size: 

                 246     164      19
 
EaeI y'GGCC_r

Cuts at:      0     282     335     429 
   Size:        282      53      94

  Fragments arranged by size: 

                 282      94      53
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      72     429 
   Size:         72     357
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     202     234     429 
   Size:        202      32     195

  Fragments arranged by size: 

                 202     195      32
 
MscI TGG'CCA

Cuts at:      0     284     429 
   Size:        284     145
 
MslI CAynn'nnrTG

Cuts at:      0     307     429 
   Size:        307     122
 
MspA1I CmG'CkG

Cuts at:      0      31     429 
   Size:         31     398
 
NsiI A_TGCA'T

Cuts at:      0      11     429 
   Size:         11     418
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     162     429 
   Size:        162     267
 
PstI C_TGCA'G

Cuts at:      0     225     429 
   Size:        225     204
 
SexAI A'CCwGG_T

Cuts at:      0     155     429 
   Size:        155     274
 
SfcI C'TryA_G

Cuts at:      0     221     429 
   Size:        221     208
 
StyI C'CwwG_G

Cuts at:      0     366     429 
   Size:        366      63
 
TatI w'GTAC_w

Cuts at:      0     111     429 
   Size:        111     318
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      67     429 
   Size:         67     362
 

 Enzymes that do cut:

    AlwNI     BaeI    BanII    BfrBI     BpmI  BsiHKAI Bsp1286I    BspMI
    BtgZI     EaeI  Eco57MI    Hin4I     MscI     MslI   MspA1I     NsiI
    PflMI     PstI    SexAI     SfcI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI