(Linear) (Six-base) MAPSORT of: KIEE6133.seq  Check: 3270  from: 1  to: 153



KIEE6133.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:25  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      17     153 
   Size:         17     136
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     153 
   Size:        153
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     114     153 
   Size:        114      39
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      62     153 
   Size:         62      91
 
EaeI y'GGCC_r

Cuts at:      0       2     153 
   Size:          2     151
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      96     153 
   Size:         96      57
 
MscI TGG'CCA

Cuts at:      0       4     153 
   Size:          4     149
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     150     153 
   Size:        150       3
 

 Enzymes that do cut:

    AlwNI     BbsI    BseRI     DrdI     EaeI     EciI     MscI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI