(Linear) (Six-base) MAPSORT of: KIEE6127.seq  Check: 9563  from: 1  to: 414



KIEE6127.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:25  ..

AccI GT'mk_AC

Cuts at:      0     401     414 
   Size:        401      13
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     414 
   Size:        322      92
 
AleI CACnn'nnGTG

Cuts at:      0     172     414 
   Size:        172     242
 
ApaI G_GGCC'C

Cuts at:      0     316     414 
   Size:        316      98
 
ApaLI G'TGCA_C

Cuts at:      0     390     414 
   Size:        390      24
 
BanII G_rGCy'C

Cuts at:      0     316     414 
   Size:        316      98
 
BbvCI CC'TCA_GC

Cuts at:      0      85     414 
   Size:         85     329
 
Bme1580I G_kGCm'C

Cuts at:      0     316     394     414 
   Size:        316      78      20

  Fragments arranged by size: 

                 316      78      20
 
Bpu10I CC'TnA_GC

Cuts at:      0      85     414 
   Size:         85     329
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     228     258     414 
   Size:        228      30     156

  Fragments arranged by size: 

                 228     156      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     223     232     414 
   Size:        223       9     182

  Fragments arranged by size: 

                 223     182       9
 
BseYI C'CCAG_C

Cuts at:      0      66     414 
   Size:         66     348
 
BsiEI CG_ry'CG

Cuts at:      0      37     414 
   Size:         37     377
 
BsiHKAI G_wGCw'C

Cuts at:      0     394     414 
   Size:        394      20
 
Bsp1286I G_dGCh'C

Cuts at:      0     316     394     414 
   Size:        316      78      20

  Fragments arranged by size: 

                 316      78      20
 
BssSI C'ACGA_G

Cuts at:      0     324     414 
   Size:        324      90
 
BtsI GCAGTG_nn'

Cuts at:      0     128     414 
   Size:        128     286
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     391     414 
   Size:        391      23
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     414 
   Size:        322      92
 
EcoO109I rG'GnC_Cy

Cuts at:      0     239     312     313     414 
   Size:        239      73       1     101

  Fragments arranged by size: 

                 239     101      73       1
 
FspI TGC'GCA

Cuts at:      0     178     414 
   Size:        178     236
 
FspAI rTGC'GCAy

Cuts at:      0     178     414 
   Size:        178     236
 
MslI CAynn'nnrTG

Cuts at:      0     172     414 
   Size:        172     242
 
PspOMI G'GGCC_C

Cuts at:      0     312     414 
   Size:        312     102
 
PstI C_TGCA'G

Cuts at:      0     108     414 
   Size:        108     306
 
PvuI CG_AT'CG

Cuts at:      0      37     414 
   Size:         37     377
 
SfcI C'TryA_G

Cuts at:      0     104     414 
   Size:        104     310
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     120     414 
   Size:        120     294
 

 Enzymes that do cut:

     AccI     AcuI     AleI     ApaI    ApaLI    BanII    BbvCI Bme1580I
   Bpu10I    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BssSI
     BtsI     EciI  Eco57MI EcoO109I     FspI    FspAI     MslI   PspOMI
     PstI     PvuI     SfcI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI