Restriction map for
pFN21AE6125
(Linear) (Six-base) MAPSORT of: KIEE6125.seq Check: 1985 from: 1 to: 528
KIEE6125.orf
With 182 enzymes: SgfI *
December 5, 2014 13:25 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 528
Size: 277 251
AleI CACnn'nnGTG
Cuts at: 0 244 528
Size: 244 284
AlwNI CAG_nnn'CTG
Cuts at: 0 60 108 528
Size: 60 48 420
Fragments arranged by size:
420 60 48
ApaI G_GGCC'C
Cuts at: 0 30 190 528
Size: 30 160 338
Fragments arranged by size:
338 160 30
BanI G'GyrC_C
Cuts at: 0 235 528
Size: 235 293
BanII G_rGCy'C
Cuts at: 0 30 190 528
Size: 30 160 338
Fragments arranged by size:
338 160 30
Bme1580I G_kGCm'C
Cuts at: 0 30 190 330 528
Size: 30 160 140 198
Fragments arranged by size:
198 160 140 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 366 528
Size: 366 162
BseYI C'CCAG_C
Cuts at: 0 67 528
Size: 67 461
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 528
Size: 442 86
Bsp1286I G_dGCh'C
Cuts at: 0 30 190 330 528
Size: 30 160 140 198
Fragments arranged by size:
198 160 140 30
BsrBI CCG'CTC
Cuts at: 0 37 528
Size: 37 491
BstXI CCAn_nnnn'nTGG
Cuts at: 0 246 528
Size: 246 282
BtgI C'CryG_G
Cuts at: 0 43 158 528
Size: 43 115 370
Fragments arranged by size:
370 115 43
DrdI GACnn_nn'nnGTC
Cuts at: 0 69 162 528
Size: 69 93 366
Fragments arranged by size:
366 93 69
EaeI y'GGCC_r
Cuts at: 0 32 464 528
Size: 32 432 64
Fragments arranged by size:
432 64 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 528
Size: 277 251
EcoNI CCTnn'n_nnAGG
Cuts at: 0 194 528
Size: 194 334
EcoO109I rG'GnC_Cy
Cuts at: 0 27 57 71 187 528
Size: 27 30 14 116 341
Fragments arranged by size:
341 116 30 27 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 82 108 114 140 528
Size: 82 26 6 26 388
Fragments arranged by size:
388 82 26 26 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 528
Size: 251 277
MslI CAynn'nnrTG
Cuts at: 0 244 528
Size: 244 284
MspA1I CmG'CkG
Cuts at: 0 24 67 108 372 528
Size: 24 43 41 264 156
Fragments arranged by size:
264 156 43 41 24
NcoI C'CATG_G
Cuts at: 0 158 528
Size: 158 370
PasI CC'CwG_GG
Cuts at: 0 168 528
Size: 168 360
PfoI T'CCnGG_A
Cuts at: 0 508 528
Size: 508 20
PpuMI rG'GwC_Cy
Cuts at: 0 57 71 528
Size: 57 14 457
Fragments arranged by size:
457 57 14
PspOMI G'GGCC_C
Cuts at: 0 26 186 528
Size: 26 160 342
Fragments arranged by size:
342 160 26
PstI C_TGCA'G
Cuts at: 0 131 528
Size: 131 397
PvuII CAG'CTG
Cuts at: 0 67 108 528
Size: 67 41 420
Fragments arranged by size:
420 67 41
SanDI GG'GwC_CC
Cuts at: 0 71 528
Size: 71 457
SexAI A'CCwGG_T
Cuts at: 0 440 528
Size: 440 88
SfcI C'TryA_G
Cuts at: 0 127 367 528
Size: 127 240 161
Fragments arranged by size:
240 161 127
StyI C'CwwG_G
Cuts at: 0 158 528
Size: 158 370
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 76 528
Size: 76 452
TatI w'GTAC_w
Cuts at: 0 51 528
Size: 51 477
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 165 438 528
Size: 165 273 90
Fragments arranged by size:
273 165 90
Enzymes that do cut:
AcuI AleI AlwNI ApaI BanI BanII Bme1580I BmrI
BseYI BsgI Bsp1286I BsrBI BstXI BtgI DrdI EaeI
Eco57MI EcoNI EcoO109I Hin4I MmeI MslI MspA1I NcoI
PasI PfoI PpuMI PspOMI PstI PvuII SanDI SexAI
SfcI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII EagI EarI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI