(Linear) (Six-base) MAPSORT of: KIEE6121.seq  Check: 9796  from: 1  to: 405



KIEE6121.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     375     405 
   Size:        130     245      30

  Fragments arranged by size: 

                 245     130      30
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     381     405 
   Size:        381      24
 
BlpI GC'TnA_GC

Cuts at:      0     163     405 
   Size:        163     242
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     339     352     405 
   Size:        339      13      53

  Fragments arranged by size: 

                 339      53      13
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     229     283     286     405 
   Size:        229      54       3     119

  Fragments arranged by size: 

                 229     119      54       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     310     405 
   Size:        208     102      95

  Fragments arranged by size: 

                 208     102      95
 
BsiHKAI G_wGCw'C

Cuts at:      0     278     405 
   Size:        278     127
 
Bsp1286I G_dGCh'C

Cuts at:      0     278     405 
   Size:        278     127
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      68     375     405 
   Size:         68     307      30

  Fragments arranged by size: 

                 307      68      30
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     370     405 
   Size:        370      35
 
Bsu36I CC'TnA_GG

Cuts at:      0     223     405 
   Size:        223     182
 
BtsI GCAGTG_nn'

Cuts at:      0     380     405 
   Size:        380      25
 
DraIII CAC_nnn'GTG

Cuts at:      0     282     405 
   Size:        282     123
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     339     352     375     405 
   Size:        130     209      13      23      30

  Fragments arranged by size: 

                 209     130      30      23      13
 
EcoO109I rG'GnC_Cy

Cuts at:      0     219     303     405 
   Size:        219      84     102

  Fragments arranged by size: 

                 219     102      84
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     333     365     405 
   Size:        333      32      40

  Fragments arranged by size: 

                 333      40      32
 
MslI CAynn'nnrTG

Cuts at:      0     151     405 
   Size:        151     254
 
PpuMI rG'GwC_Cy

Cuts at:      0     219     303     405 
   Size:        219      84     102

  Fragments arranged by size: 

                 219     102      84
 
SexAI A'CCwGG_T

Cuts at:      0     278     405 
   Size:        278     127
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     361     393     405 
   Size:        361      32      12

  Fragments arranged by size: 

                 361      32      12
 

 Enzymes that do cut:

     AcuI     BbsI     BlpI     BpmI    BseRI     BsgI  BsiHKAI Bsp1286I
    BspMI   BstAPI   Bsu36I     BtsI   DraIII  Eco57MI EcoO109I    Hin4I
     MslI    PpuMI    SexAI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI