(Linear) (Six-base) MAPSORT of: KIEE6120.seq  Check: 6200  from: 1  to: 342



KIEE6120.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:24  ..

AccI GT'mk_AC

Cuts at:      0     224     342 
   Size:        224     118
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      32      66     342 
   Size:         32      34     276

  Fragments arranged by size: 

                 276      34      32
 
Bme1580I G_kGCm'C

Cuts at:      0     221     342 
   Size:        221     121
 
BsaWI w'CCGG_w

Cuts at:      0     260     342 
   Size:        260      82
 
BseYI C'CCAG_C

Cuts at:      0     163     342 
   Size:        163     179
 
Bsp1286I G_dGCh'C

Cuts at:      0     221     342 
   Size:        221     121
 
BtgI C'CryG_G

Cuts at:      0     269     342 
   Size:        269      73
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      32     342 
   Size:         32     310
 
HaeII r_GCGC'y

Cuts at:      0     277     342 
   Size:        277      65
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     278     310     342 
   Size:        278      32      32

  Fragments arranged by size: 

                 278      32      32
 
NcoI C'CATG_G

Cuts at:      0     269     342 
   Size:        269      73
 
SfcI C'TryA_G

Cuts at:      0      60     342 
   Size:         60     282
 
StuI AGG'CCT

Cuts at:      0      58     239     342 
   Size:         58     181     103

  Fragments arranged by size: 

                 181     103      58
 
StyI C'CwwG_G

Cuts at:      0     269     342 
   Size:        269      73
 
TatI w'GTAC_w

Cuts at:      0       2     342 
   Size:          2     340
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     208     342 
   Size:        208     134
 

 Enzymes that do cut:

     AccI     BcgI Bme1580I    BsaWI    BseYI Bsp1286I     BtgI    CspCI
    HaeII    Hin4I     NcoI     SfcI     StuI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI