(Linear) (Six-base) MAPSORT of: KIEE6116.seq  Check: 7983  from: 1  to: 438



KIEE6116.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:24  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     274     438 
   Size:        274     164
 
ApaLI G'TGCA_C

Cuts at:      0     136     438 
   Size:        136     302
 
ApoI r'AATT_y

Cuts at:      0     418     438 
   Size:        418      20
 
BanI G'GyrC_C

Cuts at:      0      67     324     438 
   Size:         67     257     114

  Fragments arranged by size: 

                 257     114      67
 
BbeI G_GCGC'C

Cuts at:      0      71     438 
   Size:         71     367
 
BbvCI CC'TCA_GC

Cuts at:      0      77     438 
   Size:         77     361
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     394     438 
   Size:        394      44
 
BfrBI ATG'CAT

Cuts at:      0     237     438 
   Size:        237     201
 
Bme1580I G_kGCm'C

Cuts at:      0      63     140     438 
   Size:         63      77     298

  Fragments arranged by size: 

                 298      77      63
 
Bpu10I CC'TnA_GC

Cuts at:      0      77     438 
   Size:         77     361
 
BsaHI Gr'CG_yC

Cuts at:      0      68     438 
   Size:         68     370
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94     438 
   Size:         94     344
 
BsiHKAI G_wGCw'C

Cuts at:      0     140     438 
   Size:        140     298
 
Bsp1286I G_dGCh'C

Cuts at:      0      63     140     438 
   Size:         63      77     298

  Fragments arranged by size: 

                 298      77      63
 
BstYI r'GATC_y

Cuts at:      0      10     438 
   Size:         10     428
 
BtsI GCAGTG_nn'

Cuts at:      0     430     438 
   Size:        430       8
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     194     229     438 
   Size:        194      35     209

  Fragments arranged by size: 

                 209     194      35
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     208     438 
   Size:        208     230
 
HaeII r_GCGC'y

Cuts at:      0      71     438 
   Size:         71     367
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       2      34     438 
   Size:          2      32     404

  Fragments arranged by size: 

                 404      32       2
 
KasI G'GCGC_C

Cuts at:      0      67     438 
   Size:         67     371
 
MslI CAynn'nnrTG

Cuts at:      0     242     438 
   Size:        242     196
 
NarI GG'CG_CC

Cuts at:      0      68     438 
   Size:         68     370
 
NsiI A_TGCA'T

Cuts at:      0     239     438 
   Size:        239     199
 
PstI C_TGCA'G

Cuts at:      0      90     437     438 
   Size:         90     347       1

  Fragments arranged by size: 

                 347      90       1
 
SfcI C'TryA_G

Cuts at:      0      86     433     438 
   Size:         86     347       5

  Fragments arranged by size: 

                 347      86       5
 
SfoI GGC'GCC

Cuts at:      0      69     438 
   Size:         69     369
 
XbaI T'CTAG_A

Cuts at:      0     165     438 
   Size:        165     273
 

 Enzymes that do cut:

     AlfI    ApaLI     ApoI     BanI     BbeI    BbvCI    BciVI    BfrBI
 Bme1580I   Bpu10I    BsaHI    BseRI  BsiHKAI Bsp1286I    BstYI     BtsI
    CspCI     EciI    HaeII    Hin4I     KasI     MslI     NarI     NsiI
     PstI     SfcI     SfoI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI     ApaI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbsI
     BcgI     BclI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
     DraI   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI