(Linear) (Six-base) MAPSORT of: KIEE6110.seq  Check: 8302  from: 1  to: 483



KIEE6110.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370     483 
   Size:        370     113
 
BanII G_rGCy'C

Cuts at:      0     407     483 
   Size:        407      76
 
BbvCI CC'TCA_GC

Cuts at:      0      92     483 
   Size:         92     391
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     481     483 
   Size:        481       2
 
Bpu10I CC'TnA_GC

Cuts at:      0      92     483 
   Size:         92     391
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     370     483 
   Size:        370     113
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     274     304     483 
   Size:        274      30     179

  Fragments arranged by size: 

                 274     179      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     178     295     298     415     483 
   Size:        178     117       3     117      68

  Fragments arranged by size: 

                 178     117     117      68       3
 
BseYI C'CCAG_C

Cuts at:      0      84     120     141     483 
   Size:         84      36      21     342

  Fragments arranged by size: 

                 342      84      36      21
 
BsiEI CG_ry'CG

Cuts at:      0     438     483 
   Size:        438      45
 
BsiHKAI G_wGCw'C

Cuts at:      0     407     483 
   Size:        407      76
 
Bsp1286I G_dGCh'C

Cuts at:      0     407     483 
   Size:        407      76
 
BspHI T'CATG_A

Cuts at:      0     236     407     467     483 
   Size:        236     171      60      16

  Fragments arranged by size: 

                 236     171      60      16
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     120     483 
   Size:        120     363
 
Bsu36I CC'TnA_GG

Cuts at:      0     161     483 
   Size:        161     322
 
ClaI AT'CG_AT

Cuts at:      0     182     483 
   Size:        182     301
 
EaeI y'GGCC_r

Cuts at:      0     435     483 
   Size:        435      48
 
EagI C'GGCC_G

Cuts at:      0     435     483 
   Size:        435      48
 
EarI CTCTTCn'nnn_

Cuts at:      0      35     304     483 
   Size:         35     269     179

  Fragments arranged by size: 

                 269     179      35
 
EcoICRI GAG'CTC

Cuts at:      0     405     483 
   Size:        405      78
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370     481     483 
   Size:        370     111       2

  Fragments arranged by size: 

                 370     111       2
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      84     483 
   Size:         84     399
 
HaeII r_GCGC'y

Cuts at:      0      92     483 
   Size:         92     391
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     274     306     483 
   Size:        274      32     177

  Fragments arranged by size: 

                 274     177      32
 
MslI CAynn'nnrTG

Cuts at:      0      79     483 
   Size:         79     404
 
MspA1I CmG'CkG

Cuts at:      0      33     206     483 
   Size:         33     173     277

  Fragments arranged by size: 

                 277     173      33
 
NspI r_CATG'y

Cuts at:      0     309     483 
   Size:        309     174
 
PfoI T'CCnGG_A

Cuts at:      0     458     483 
   Size:        458      25
 
PstI C_TGCA'G

Cuts at:      0     173     365     483 
   Size:        173     192     118

  Fragments arranged by size: 

                 192     173     118
 
PvuII CAG'CTG

Cuts at:      0      33     483 
   Size:         33     450
 
SacI G_AGCT'C

Cuts at:      0     407     483 
   Size:        407      76
 
SapI GCTCTTCn'nnn_

Cuts at:      0      35     483 
   Size:         35     448
 
SfcI C'TryA_G

Cuts at:      0     169     361     368     483 
   Size:        169     192       7     115

  Fragments arranged by size: 

                 192     169     115       7
 
StyI C'CwwG_G

Cuts at:      0     113     483 
   Size:        113     370
 

 Enzymes that do cut:

     AcuI    BanII    BbvCI     BpmI   Bpu10I     BsaI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspHI    BstXI   Bsu36I     ClaI
     EaeI     EagI     EarI  EcoICRI  Eco57MI     FalI    HaeII    Hin4I
     MslI   MspA1I     NspI     PfoI     PstI    PvuII     SacI     SapI
     SfcI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I     BtgI    BtgZI     BtsI
    CspCI     DraI   DraIII     DrdI     EciI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI