Restriction map for
pFN21AE6109
(Linear) (Six-base) MAPSORT of: KIEE6109.seq Check: 9841 from: 1 to: 450
KIEE6109.orf
With 182 enzymes: SgfI *
December 5, 2014 13:24 ..
AvaI C'yCGr_G
Cuts at: 0 44 56 450
Size: 44 12 394
Fragments arranged by size:
394 44 12
BanI G'GyrC_C
Cuts at: 0 7 450
Size: 7 443
BanII G_rGCy'C
Cuts at: 0 257 450
Size: 257 193
BbeI G_GCGC'C
Cuts at: 0 11 450
Size: 11 439
BciVI GTATCCnnnnn_n'
Cuts at: 0 331 450
Size: 331 119
BmrI ACTGGGnnnn_n'
Cuts at: 0 418 442 450
Size: 418 24 8
Fragments arranged by size:
418 24 8
BsaHI Gr'CG_yC
Cuts at: 0 8 450
Size: 8 442
BsaWI w'CCGG_w
Cuts at: 0 423 450
Size: 423 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 450
Size: 163 287
BseYI C'CCAG_C
Cuts at: 0 3 164 450
Size: 3 161 286
Fragments arranged by size:
286 161 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 450
Size: 52 398
Bsp1286I G_dGCh'C
Cuts at: 0 257 450
Size: 257 193
BspEI T'CCGG_A
Cuts at: 0 423 450
Size: 423 27
BsrBI CCG'CTC
Cuts at: 0 13 450
Size: 13 437
BtgI C'CryG_G
Cuts at: 0 95 126 450
Size: 95 31 324
Fragments arranged by size:
324 95 31
DraI TTT'AAA
Cuts at: 0 401 450
Size: 401 49
EaeI y'GGCC_r
Cuts at: 0 32 450
Size: 32 418
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 7 63 450
Size: 7 56 387
Fragments arranged by size:
387 56 7
EcoO109I rG'GnC_Cy
Cuts at: 0 349 450
Size: 349 101
HaeII r_GCGC'y
Cuts at: 0 11 450
Size: 11 439
KasI G'GCGC_C
Cuts at: 0 7 450
Size: 7 443
MspA1I CmG'CkG
Cuts at: 0 23 450
Size: 23 427
NarI GG'CG_CC
Cuts at: 0 8 450
Size: 8 442
NcoI C'CATG_G
Cuts at: 0 126 450
Size: 126 324
NspI r_CATG'y
Cuts at: 0 287 450
Size: 287 163
PfoI T'CCnGG_A
Cuts at: 0 380 450
Size: 380 70
PpuMI rG'GwC_Cy
Cuts at: 0 349 450
Size: 349 101
PspXI vC'TCGA_Gb
Cuts at: 0 56 450
Size: 56 394
SfoI GGC'GCC
Cuts at: 0 9 450
Size: 9 441
SmaI CCC'GGG
Cuts at: 0 46 450
Size: 46 404
SmlI C'TyrA_G
Cuts at: 0 56 450
Size: 56 394
SphI G_CATG'C
Cuts at: 0 287 450
Size: 287 163
StuI AGG'CCT
Cuts at: 0 62 450
Size: 62 388
StyI C'CwwG_G
Cuts at: 0 126 450
Size: 126 324
TatI w'GTAC_w
Cuts at: 0 310 450
Size: 310 140
XhoI C'TCGA_G
Cuts at: 0 56 450
Size: 56 394
XmaI C'CCGG_G
Cuts at: 0 44 450
Size: 44 406
Enzymes that do cut:
AvaI BanI BanII BbeI BciVI BmrI BsaHI BsaWI
BseRI BseYI BsgI Bsp1286I BspEI BsrBI BtgI DraI
EaeI EciI EcoO109I HaeII KasI MspA1I NarI NcoI
NspI PfoI PpuMI PspXI SfoI SmaI SmlI SphI
StuI StyI TatI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraIII DrdI EagI EarI EcoICRI Eco57MI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TaqII TstI Tth111I XbaI XcmI XmnI ZraI