(Linear) (Six-base) MAPSORT of: KIEE6104.seq  Check: 3020  from: 1  to: 393



KIEE6104.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:23  ..

BglII A'GATC_T

Cuts at:      0     306     393 
   Size:        306      87
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      82     116     393 
   Size:         82      34     277

  Fragments arranged by size: 

                 277      82      34
 
BsaAI yAC'GTr

Cuts at:      0     250     393 
   Size:        250     143
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     340     393 
   Size:        340      53
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     161     272     393 
   Size:        161     111     121

  Fragments arranged by size: 

                 161     121     111
 
BstYI r'GATC_y

Cuts at:      0     306     393 
   Size:        306      87
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     307     339     393 
   Size:        307      32      54

  Fragments arranged by size: 

                 307      54      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     176     393 
   Size:        176     217
 
PmlI CAC'GTG

Cuts at:      0     250     393 
   Size:        250     143
 
SspI AAT'ATT

Cuts at:      0      88     393 
   Size:         88     305
 

 Enzymes that do cut:

    BglII     BmrI    BsaAI    BseRI     BsgI    BstYI    Hin4I     MmeI
     PmlI     SspI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI