(Linear) (Six-base) MAPSORT of: KIEE6103.seq  Check: 5864  from: 1  to: 405



KIEE6103.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:23  ..

AclI AA'CG_TT

Cuts at:      0     316     405 
   Size:        316      89
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     405 
   Size:         73     332
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      59     405 
   Size:         59     346
 
AlwNI CAG_nnn'CTG

Cuts at:      0      70     405 
   Size:         70     335
 
AseI AT'TA_AT

Cuts at:      0     212     405 
   Size:        212     193
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      58     405 
   Size:         58     347
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     405 
   Size:         28     377
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     356     386     405 
   Size:        356      30      19

  Fragments arranged by size: 

                 356      30      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     405 
   Size:         55     350
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     344     405 
   Size:        344      61
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28      73     405 
   Size:         28      45     332

  Fragments arranged by size: 

                 332      45      28
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     222     405 
   Size:        222     183
 
StyI C'CwwG_G

Cuts at:      0     132     405 
   Size:        132     273
 
TatI w'GTAC_w

Cuts at:      0     138     405 
   Size:        138     267
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     356     388     405 
   Size:        356      32      17

  Fragments arranged by size: 

                 356      32      17
 
XmnI GAAnn'nnTTC

Cuts at:      0     113     405 
   Size:        113     292
 

 Enzymes that do cut:

     AclI     AcuI     AlfI    AlwNI     AseI     BmrI     BpmI    BsaXI
     BsgI    BtgZI  Eco57MI     FalI     StyI     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI