(Linear) (Six-base) MAPSORT of: KIEE6102.seq  Check: 4935  from: 1  to: 315



KIEE6102.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:23  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     315 
   Size:        190     125
 
BanII G_rGCy'C

Cuts at:      0     233     315 
   Size:        233      82
 
BbvCI CC'TCA_GC

Cuts at:      0      97     315 
   Size:         97     218
 
BglII A'GATC_T

Cuts at:      0     128     315 
   Size:        128     187
 
Bpu10I CC'TnA_GC

Cuts at:      0      97     315 
   Size:         97     218
 
BsaWI w'CCGG_w

Cuts at:      0     233     315 
   Size:        233      82
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     220     250     315 
   Size:        220      30      65

  Fragments arranged by size: 

                 220      65      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     100     262     315 
   Size:        100     162      53

  Fragments arranged by size: 

                 162     100      53
 
BseYI C'CCAG_C

Cuts at:      0      74     315 
   Size:         74     241
 
BsiHKAI G_wGCw'C

Cuts at:      0     233     315 
   Size:        233      82
 
Bsp1286I G_dGCh'C

Cuts at:      0     233     315 
   Size:        233      82
 
BsrBI CCG'CTC

Cuts at:      0     224     315 
   Size:        224      91
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     195     315 
   Size:        195     120
 
BstYI r'GATC_y

Cuts at:      0     128     315 
   Size:        128     187
 
Bsu36I CC'TnA_GG

Cuts at:      0     245     315 
   Size:        245      70
 
BtgI C'CryG_G

Cuts at:      0      80     315 
   Size:         80     235
 
BtsI GCAGTG_nn'

Cuts at:      0      21     315 
   Size:         21     294
 
EaeI y'GGCC_r

Cuts at:      0      44     315 
   Size:         44     271
 
EarI CTCTTCn'nnn_

Cuts at:      0     281     315 
   Size:        281      34
 
EcoICRI GAG'CTC

Cuts at:      0     231     315 
   Size:        231      84
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     315 
   Size:        190     125
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     259     315 
   Size:        259      56
 
MslI CAynn'nnrTG

Cuts at:      0     193     315 
   Size:        193     122
 
MspA1I CmG'CkG

Cuts at:      0     161     315 
   Size:        161     154
 
NcoI C'CATG_G

Cuts at:      0      80     315 
   Size:         80     235
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      81     315 
   Size:         81     234
 
PfoI T'CCnGG_A

Cuts at:      0     260     315 
   Size:        260      55
 
SacI G_AGCT'C

Cuts at:      0     233     315 
   Size:        233      82
 
StyI C'CwwG_G

Cuts at:      0      80     315 
   Size:         80     235
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      88     315 
   Size:         88     227
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     220     252     315 
   Size:        220      32      63

  Fragments arranged by size: 

                 220      63      32
 

 Enzymes that do cut:

     AcuI    BanII    BbvCI    BglII   Bpu10I    BsaWI    BsaXI    BseRI
    BseYI  BsiHKAI Bsp1286I    BsrBI    BstXI    BstYI   Bsu36I     BtgI
     BtsI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI     MslI   MspA1I
     NcoI    PflMI     PfoI     SacI     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI