(Linear) (Six-base) MAPSORT of: KIEE6101.seq  Check: 6339  from: 1  to: 309



KIEE6101.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:23  ..

AleI CACnn'nnGTG

Cuts at:      0     244     309 
   Size:        244      65
 
AlwNI CAG_nnn'CTG

Cuts at:      0     156     309 
   Size:        156     153
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      37     309 
   Size:         37     272
 
BsiHKAI G_wGCw'C

Cuts at:      0     180     309 
   Size:        180     129
 
Bsp1286I G_dGCh'C

Cuts at:      0     180     309 
   Size:        180     129
 
BstYI r'GATC_y

Cuts at:      0     134     309 
   Size:        134     175
 
EaeI y'GGCC_r

Cuts at:      0      80     236     309 
   Size:         80     156      73

  Fragments arranged by size: 

                 156      80      73
 
HaeII r_GCGC'y

Cuts at:      0      43     309 
   Size:         43     266
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     246     309 
   Size:        246      63
 
MscI TGG'CCA

Cuts at:      0      82     238     309 
   Size:         82     156      71

  Fragments arranged by size: 

                 156      82      71
 
MslI CAynn'nnrTG

Cuts at:      0     244     309 
   Size:        244      65
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      89     309 
   Size:         89     220
 
StyI C'CwwG_G

Cuts at:      0     101     309 
   Size:        101     208
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     108     309 
   Size:        108     201
 

 Enzymes that do cut:

     AleI    AlwNI     BbsI  BsiHKAI Bsp1286I    BstYI     EaeI    HaeII
     MmeI     MscI     MslI    PflMI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI