(Linear) (Six-base) MAPSORT of: KIEE6093.seq  Check: 2048  from: 1  to: 978



KIEE6093.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:23  ..

AflIII A'CryG_T

Cuts at:      0     224     978 
   Size:        224     754
 
AhdI GACnn_n'nnGTC

Cuts at:      0     751     978 
   Size:        751     227
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     482     978 
   Size:        482     496
 
ApoI r'AATT_y

Cuts at:      0      51      76     237     978 
   Size:         51      25     161     741

  Fragments arranged by size: 

                 741     161      51      25
 
BamHI G'GATC_C

Cuts at:      0     691     978 
   Size:        691     287
 
BanII G_rGCy'C

Cuts at:      0     829     978 
   Size:        829     149
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     747     978 
   Size:        747     231
 
BclI T'GATC_A

Cuts at:      0     405     978 
   Size:        405     573
 
BglII A'GATC_T

Cuts at:      0     116     978 
   Size:        116     862
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     464     749     978 
   Size:        464     285     229

  Fragments arranged by size: 

                 464     285     229
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325     904     978 
   Size:        325     579      74

  Fragments arranged by size: 

                 579     325      74
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     776     978 
   Size:        776     202
 
BseYI C'CCAG_C

Cuts at:      0     488     978 
   Size:        488     490
 
BsmI GAATG_Cn'

Cuts at:      0     382     978 
   Size:        382     596
 
Bsp1286I G_dGCh'C

Cuts at:      0     829     978 
   Size:        829     149
 
BspHI T'CATG_A

Cuts at:      0     449     978 
   Size:        449     529
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0       9     978 
   Size:          9     969
 
BsrDI GCAATG_nn'

Cuts at:      0     176     978 
   Size:        176     802
 
BsrFI r'CCGG_y

Cuts at:      0     200     978 
   Size:        200     778
 
BsrGI T'GTAC_A

Cuts at:      0     221     978 
   Size:        221     757
 
BstEII G'GTnAC_C

Cuts at:      0     885     978 
   Size:        885      93
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     288     978 
   Size:        288     690
 
BstYI r'GATC_y

Cuts at:      0     116     691     978 
   Size:        116     575     287

  Fragments arranged by size: 

                 575     287     116
 
Bsu36I CC'TnA_GG

Cuts at:      0     946     978 
   Size:        946      32
 
EarI CTCTTCn'nnn_

Cuts at:      0     130     273     901     978 
   Size:        130     143     628      77

  Fragments arranged by size: 

                 628     143     130      77
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325     904     978 
   Size:        325     579      74

  Fragments arranged by size: 

                 579     325      74
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     323     355     444     476     737     769     978 
   Size:        323      32      89      32     261      32     209

  Fragments arranged by size: 

                 323     261     209      89      32      32      32
 
HincII GTy'rAC

Cuts at:      0     294     978 
   Size:        294     684
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     654     978 
   Size:        654     324
 
MslI CAynn'nnrTG

Cuts at:      0     217     223     375     464     820     978 
   Size:        217       6     152      89     356     158

  Fragments arranged by size: 

                 356     217     158     152      89       6
 
NdeI CA'TA_TG

Cuts at:      0     147     978 
   Size:        147     831
 
NspI r_CATG'y

Cuts at:      0     228     978 
   Size:        228     750
 
PasI CC'CwG_GG

Cuts at:      0     586     978 
   Size:        586     392
 
PciI A'CATG_T

Cuts at:      0     224     978 
   Size:        224     754
 
SfcI C'TryA_G

Cuts at:      0     272     798     845     912     978 
   Size:        272     526      47      67      66

  Fragments arranged by size: 

                 526     272      67      66      47
 
StyI C'CwwG_G

Cuts at:      0      87     978 
   Size:         87     891
 
TatI w'GTAC_w

Cuts at:      0     150     221     978 
   Size:        150      71     757

  Fragments arranged by size: 

                 757     150      71
 

 Enzymes that do cut:

   AflIII     AhdI     AlfI     ApoI    BamHI    BanII     BbsI     BclI
    BglII     BmrI     BpmI     BsaI    BseYI     BsmI Bsp1286I    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BstEII    BstXI    BstYI   Bsu36I
     EarI  Eco57MI    Hin4I   HincII     MmeI     MslI     NdeI     NspI
     PasI     PciI     SfcI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BsrBI   BssHII
    BssSI   BstAPI    BstBI  BstZ17I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI