Restriction map for
pFN21AE6090
(Linear) (Six-base) MAPSORT of: KIEE6090.seq Check: 6983 from: 1 to: 975
KIEE6090.orf
With 182 enzymes: SgfI *
December 5, 2014 13:23 ..
AflIII A'CryG_T
Cuts at: 0 721 732 975
Size: 721 11 243
Fragments arranged by size:
721 243 11
AleI CACnn'nnGTG
Cuts at: 0 214 735 975
Size: 214 521 240
Fragments arranged by size:
521 240 214
BamHI G'GATC_C
Cuts at: 0 53 975
Size: 53 922
BanI G'GyrC_C
Cuts at: 0 849 975
Size: 849 126
BbsI GAAGACnn'nnnn_
Cuts at: 0 676 975
Size: 676 299
BbvCI CC'TCA_GC
Cuts at: 0 715 975
Size: 715 260
BclI T'GATC_A
Cuts at: 0 149 975
Size: 149 826
BglI GCCn_nnn'nGGC
Cuts at: 0 202 975
Size: 202 773
BglII A'GATC_T
Cuts at: 0 896 975
Size: 896 79
Bme1580I G_kGCm'C
Cuts at: 0 809 975
Size: 809 166
BmrI ACTGGGnnnn_n'
Cuts at: 0 15 975
Size: 15 960
Bpu10I CC'TnA_GC
Cuts at: 0 715 975
Size: 715 260
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 975
Size: 691 284
BsaI GGTCTCn'nnnn_
Cuts at: 0 226 975
Size: 226 749
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 975
Size: 19 30 926
Fragments arranged by size:
926 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 24 975
Size: 24 951
BseYI C'CCAG_C
Cuts at: 0 48 975
Size: 48 927
BsiHKAI G_wGCw'C
Cuts at: 0 548 975
Size: 548 427
Bsp1286I G_dGCh'C
Cuts at: 0 548 809 975
Size: 548 261 166
Fragments arranged by size:
548 261 166
BspHI T'CATG_A
Cuts at: 0 693 975
Size: 693 282
BsrDI GCAATG_nn'
Cuts at: 0 362 641 975
Size: 362 279 334
Fragments arranged by size:
362 334 279
BsrGI T'GTAC_A
Cuts at: 0 107 975
Size: 107 868
BstXI CCAn_nnnn'nTGG
Cuts at: 0 216 975
Size: 216 759
BstYI r'GATC_y
Cuts at: 0 53 896 975
Size: 53 843 79
Fragments arranged by size:
843 79 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 772 975
Size: 772 203
DrdI GACnn_nn'nnGTC
Cuts at: 0 637 975
Size: 637 338
EaeI y'GGCC_r
Cuts at: 0 194 203 377 795 975
Size: 194 9 174 418 180
Fragments arranged by size:
418 194 180 174 9
EarI CTCTTCn'nnn_
Cuts at: 0 944 975
Size: 944 31
HaeII r_GCGC'y
Cuts at: 0 407 975
Size: 407 568
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 266 298 975
Size: 266 32 677
Fragments arranged by size:
677 266 32
MscI TGG'CCA
Cuts at: 0 196 205 379 975
Size: 196 9 174 596
Fragments arranged by size:
596 196 174 9
MslI CAynn'nnrTG
Cuts at: 0 214 304 331 735 737 975
Size: 214 90 27 404 2 238
Fragments arranged by size:
404 238 214 90 27 2
NspI r_CATG'y
Cuts at: 0 725 736 975
Size: 725 11 239
Fragments arranged by size:
725 239 11
PciI A'CATG_T
Cuts at: 0 721 732 975
Size: 721 11 243
Fragments arranged by size:
721 243 11
PshAI GACnn'nnGTC
Cuts at: 0 708 975
Size: 708 267
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 202 975
Size: 202 773
SmlI C'TyrA_G
Cuts at: 0 706 975
Size: 706 269
SspI AAT'ATT
Cuts at: 0 591 920 975
Size: 591 329 55
Fragments arranged by size:
591 329 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 34 316 975
Size: 34 282 659
Fragments arranged by size:
659 282 34
TatI w'GTAC_w
Cuts at: 0 107 975
Size: 107 868
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 19 51 975
Size: 19 32 924
Fragments arranged by size:
924 32 19
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 213 975
Size: 213 762
XmnI GAAnn'nnTTC
Cuts at: 0 71 975
Size: 71 904
Enzymes that do cut:
AflIII AleI BamHI BanI BbsI BbvCI BclI BglI
BglII Bme1580I BmrI Bpu10I BpuEI BsaI BsaXI BseRI
BseYI BsiHKAI Bsp1286I BspHI BsrDI BsrGI BstXI BstYI
BtgZI DrdI EaeI EarI HaeII Hin4I MscI MslI
NspI PciI PshAI SfiI SmlI SspI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BanII BbeI
BcgI BciVI BfrBI BlpI BmgBI BmtI BplI BpmI
BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtsI ClaI CspCI
DraI DraIII EagI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI StyI SwaI Tth111I XbaI XhoI XmaI
ZraI