(Linear) (Six-base) MAPSORT of: KIEE6087.seq  Check: 7168  from: 1  to: 975



KIEE6087.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:23  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       7     975 
   Size:          7     968
 
AleI CACnn'nnGTG

Cuts at:      0     877     975 
   Size:        877      98
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      55     975 
   Size:         55     920
 
ApoI r'AATT_y

Cuts at:      0      70     962     975 
   Size:         70     892      13

  Fragments arranged by size: 

                 892      70      13
 
BanI G'GyrC_C

Cuts at:      0     934     975 
   Size:        934      41
 
BanII G_rGCy'C

Cuts at:      0     170     975 
   Size:        170     805
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      10     975 
   Size:         10     965
 
BclI T'GATC_A

Cuts at:      0     617     975 
   Size:        617     358
 
BfrBI ATG'CAT

Cuts at:      0      42     975 
   Size:         42     933
 
Bme1580I G_kGCm'C

Cuts at:      0     939     975 
   Size:        939      36
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     933     975 
   Size:        933      42
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     106     816     975 
   Size:        106     710     159

  Fragments arranged by size: 

                 710     159     106
 
Bsp1286I G_dGCh'C

Cuts at:      0     170     939     975 
   Size:        170     769      36

  Fragments arranged by size: 

                 769     170      36
 
BssSI C'ACGA_G

Cuts at:      0     480     975 
   Size:        480     495
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     282     975 
   Size:        282     693
 
Bsu36I CC'TnA_GG

Cuts at:      0     839     975 
   Size:        839     136
 
BtgI C'CryG_G

Cuts at:      0     389     975 
   Size:        389     586
 
DraIII CAC_nnn'GTG

Cuts at:      0     408     975 
   Size:        408     567
 
EarI CTCTTCn'nnn_

Cuts at:      0     226     544     975 
   Size:        226     318     431

  Fragments arranged by size: 

                 431     318     226
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       7     975 
   Size:          7     968
 
EcoRI G'AATT_C

Cuts at:      0      70     975 
   Size:         70     905
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     238     270     975 
   Size:        238      32     705

  Fragments arranged by size: 

                 705     238      32
 
MfeI C'AATT_G

Cuts at:      0     532     776     975 
   Size:        532     244     199

  Fragments arranged by size: 

                 532     244     199
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     116     287     975 
   Size:        116     171     688

  Fragments arranged by size: 

                 688     171     116
 
MslI CAynn'nnrTG

Cuts at:      0     175     211     337     877     975 
   Size:        175      36     126     540      98

  Fragments arranged by size: 

                 540     175     126      98      36
 
NcoI C'CATG_G

Cuts at:      0     389     975 
   Size:        389     586
 
NsiI A_TGCA'T

Cuts at:      0      44     975 
   Size:         44     931
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     408     975 
   Size:        408     567
 
SapI GCTCTTCn'nnn_

Cuts at:      0     544     975 
   Size:        544     431
 
SfcI C'TryA_G

Cuts at:      0     803     906     975 
   Size:        803     103      69

  Fragments arranged by size: 

                 803     103      69
 
SmlI C'TyrA_G

Cuts at:      0     912     975 
   Size:        912      63
 
SspI AAT'ATT

Cuts at:      0     291     498     975 
   Size:        291     207     477

  Fragments arranged by size: 

                 477     291     207
 
StyI C'CwwG_G

Cuts at:      0     389     975 
   Size:        389     586
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     900     975 
   Size:        900      75
 
TatI w'GTAC_w

Cuts at:      0     341     672     812     975 
   Size:        341     331     140     163

  Fragments arranged by size: 

                 341     331     163     140
 
XmnI GAAnn'nnTTC

Cuts at:      0      74     748     898     963     975 
   Size:         74     674     150      65      12

  Fragments arranged by size: 

                 674     150      74      65      12
 

 Enzymes that do cut:

     AcuI     AleI     AlfI     ApoI     BanI    BanII     BbsI     BclI
    BfrBI Bme1580I    BpuEI    BseRI Bsp1286I    BssSI    BstXI   Bsu36I
     BtgI   DraIII     EarI  Eco57MI    EcoRI    Hin4I     MfeI     MmeI
     MslI     NcoI     NsiI    PflMI     SapI     SfcI     SmlI     SspI
     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstYI  BstZ17I    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MluI     MscI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI