Restriction map for
pFN21AE6085
(Linear) (Six-base) MAPSORT of: KIEE6085.seq Check: 660 from: 1 to: 972
KIEE6085.orf
With 182 enzymes: SgfI *
December 5, 2014 13:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 766 972
Size: 766 206
AleI CACnn'nnGTG
Cuts at: 0 773 972
Size: 773 199
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 301 333 972
Size: 301 32 639
Fragments arranged by size:
639 301 32
BanII G_rGCy'C
Cuts at: 0 212 812 972
Size: 212 600 160
Fragments arranged by size:
600 212 160
BbsI GAAGACnn'nnnn_
Cuts at: 0 780 972
Size: 780 192
BciVI GTATCCnnnnn_n'
Cuts at: 0 94 972
Size: 94 878
BglII A'GATC_T
Cuts at: 0 929 972
Size: 929 43
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 741 972
Size: 741 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 727 972
Size: 511 216 245
Fragments arranged by size:
511 245 216
BsaBI GATnn'nnATC
Cuts at: 0 301 972
Size: 301 671
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 301 331 972
Size: 301 30 641
Fragments arranged by size:
641 301 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 249 838 972
Size: 249 589 134
Fragments arranged by size:
589 249 134
BseYI C'CCAG_C
Cuts at: 0 11 828 972
Size: 11 817 144
Fragments arranged by size:
817 144 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 972
Size: 562 410
Bsp1286I G_dGCh'C
Cuts at: 0 212 812 972
Size: 212 600 160
Fragments arranged by size:
600 212 160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 766 972
Size: 766 206
BsrDI GCAATG_nn'
Cuts at: 0 424 972
Size: 424 548
BstEII G'GTnAC_C
Cuts at: 0 160 972
Size: 160 812
BstYI r'GATC_y
Cuts at: 0 929 972
Size: 929 43
BtgI C'CryG_G
Cuts at: 0 365 389 972
Size: 365 24 583
Fragments arranged by size:
583 365 24
BtsI GCAGTG_nn'
Cuts at: 0 511 972
Size: 511 461
DraIII CAC_nnn'GTG
Cuts at: 0 964 972
Size: 964 8
DrdI GACnn_nn'nnGTC
Cuts at: 0 616 972
Size: 616 356
EaeI y'GGCC_r
Cuts at: 0 149 227 281 386 410 461 972
Size: 149 78 54 105 24 51 511
Fragments arranged by size:
511 149 105 78 54 51 24
EarI CTCTTCn'nnn_
Cuts at: 0 226 643 679 972
Size: 226 417 36 293
Fragments arranged by size:
417 293 226 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 729 972
Size: 729 243
EcoO109I rG'GnC_Cy
Cuts at: 0 97 942 972
Size: 97 845 30
Fragments arranged by size:
845 97 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 270 302 626 658 972
Size: 270 32 324 32 314
Fragments arranged by size:
324 314 270 32 32
HincII GTy'rAC
Cuts at: 0 897 972
Size: 897 75
MscI TGG'CCA
Cuts at: 0 151 229 283 388 412 463 972
Size: 151 78 54 105 24 51 509
Fragments arranged by size:
509 151 105 78 54 51 24
MslI CAynn'nnrTG
Cuts at: 0 187 661 773 972
Size: 187 474 112 199
Fragments arranged by size:
474 199 187 112
MspA1I CmG'CkG
Cuts at: 0 376 972
Size: 376 596
NcoI C'CATG_G
Cuts at: 0 389 972
Size: 389 583
PasI CC'CwG_GG
Cuts at: 0 156 972
Size: 156 816
PflMI CCAn_nnn'nTGG
Cuts at: 0 18 972
Size: 18 954
PfoI T'CCnGG_A
Cuts at: 0 305 972
Size: 305 667
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 301 333 972
Size: 301 32 639
Fragments arranged by size:
639 301 32
PpuMI rG'GwC_Cy
Cuts at: 0 942 972
Size: 942 30
PshAI GACnn'nnGTC
Cuts at: 0 172 972
Size: 172 800
PvuII CAG'CTG
Cuts at: 0 376 972
Size: 376 596
SfcI C'TryA_G
Cuts at: 0 890 972
Size: 890 82
SmlI C'TyrA_G
Cuts at: 0 526 742 972
Size: 526 216 230
Fragments arranged by size:
526 230 216
StyI C'CwwG_G
Cuts at: 0 146 389 972
Size: 146 243 583
Fragments arranged by size:
583 243 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 437 649 820 972
Size: 437 212 171 152
Fragments arranged by size:
437 212 171 152
TatI w'GTAC_w
Cuts at: 0 454 604 972
Size: 454 150 368
Fragments arranged by size:
454 368 150
Tth111I GACn'n_nGTC
Cuts at: 0 250 972
Size: 250 722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 276 621 972
Size: 276 345 351
Fragments arranged by size:
351 345 276
Enzymes that do cut:
AarI AleI AloI BanII BbsI BciVI BglII BplI
BpuEI BsaBI BsaXI BseRI BseYI BsgI Bsp1286I BspMI
BsrDI BstEII BstYI BtgI BtsI DraIII DrdI EaeI
EarI EcoNI EcoO109I Hin4I HincII MscI MslI MspA1I
NcoI PasI PflMI PfoI PpiI PpuMI PshAI PvuII
SfcI SmlI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BclI BfrBI BglI BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI EagI EciI EcoICRI
Eco57MI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
XbaI XhoI XmaI XmnI ZraI