(Linear) (Six-base) MAPSORT of: KIEE6082.seq  Check: 5094  from: 1  to: 972



KIEE6082.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     754     972 
   Size:        754     218
 
AflIII A'CryG_T

Cuts at:      0      93     569     972 
   Size:         93     476     403

  Fragments arranged by size: 

                 476     403      93
 
AhdI GACnn_n'nnGTC

Cuts at:      0     570     972 
   Size:        570     402
 
AleI CACnn'nnGTG

Cuts at:      0     352     972 
   Size:        352     620
 
AlwNI CAG_nnn'CTG

Cuts at:      0     746     972 
   Size:        746     226
 
BlpI GC'TnA_GC

Cuts at:      0     458     972 
   Size:        458     514
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     676     972 
   Size:        676     296
 
BsaAI yAC'GTr

Cuts at:      0     557     972 
   Size:        557     415
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      38     193     972 
   Size:         38     155     779

  Fragments arranged by size: 

                 779     155      38
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     102     231     732     972 
   Size:        102     129     501     240

  Fragments arranged by size: 

                 501     240     129     102
 
BsiHKAI G_wGCw'C

Cuts at:      0     371     972 
   Size:        371     601
 
BsmI GAATG_Cn'

Cuts at:      0     790     972 
   Size:        790     182
 
Bsp1286I G_dGCh'C

Cuts at:      0     371     972 
   Size:        371     601
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     253     754     958     972 
   Size:        253     501     204      14

  Fragments arranged by size: 

                 501     253     204      14
 
BsrDI GCAATG_nn'

Cuts at:      0     446     972 
   Size:        446     526
 
BsrGI T'GTAC_A

Cuts at:      0     125     972 
   Size:        125     847
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     817     972 
   Size:        817     155
 
BstYI r'GATC_y

Cuts at:      0     101     972 
   Size:        101     871
 
Bsu36I CC'TnA_GG

Cuts at:      0     475     972 
   Size:        475     497
 
BtsI GCAGTG_nn'

Cuts at:      0     749     972 
   Size:        749     223
 
EaeI y'GGCC_r

Cuts at:      0     213     972 
   Size:        213     759
 
EarI CTCTTCn'nnn_

Cuts at:      0     717     848     972 
   Size:        717     131     124

  Fragments arranged by size: 

                 717     131     124
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     474     972 
   Size:        474     498
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     556     588     972 
   Size:        556      32     384

  Fragments arranged by size: 

                 556     384      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     972 
   Size:         55     917
 
MscI TGG'CCA

Cuts at:      0     215     972 
   Size:        215     757
 
MslI CAynn'nnrTG

Cuts at:      0     352     376     853     972 
   Size:        352      24     477     119

  Fragments arranged by size: 

                 477     352     119      24
 
NspI r_CATG'y

Cuts at:      0      97     573     972 
   Size:         97     476     399

  Fragments arranged by size: 

                 476     399      97
 
PciI A'CATG_T

Cuts at:      0      93     569     972 
   Size:         93     476     403

  Fragments arranged by size: 

                 476     403      93
 
SexAI A'CCwGG_T

Cuts at:      0      75     972 
   Size:         75     897
 
SfcI C'TryA_G

Cuts at:      0     575     972 
   Size:        575     397
 
SmlI C'TyrA_G

Cuts at:      0     691     972 
   Size:        691     281
 
SnaBI TAC'GTA

Cuts at:      0     557     972 
   Size:        557     415
 
SspI AAT'ATT

Cuts at:      0     543     972 
   Size:        543     429
 
StyI C'CwwG_G

Cuts at:      0     452     972 
   Size:        452     520
 
TatI w'GTAC_w

Cuts at:      0      90     125     972 
   Size:         90      35     847

  Fragments arranged by size: 

                 847      90      35
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     768     800     972 
   Size:        768      32     172

  Fragments arranged by size: 

                 768     172      32
 
XbaI T'CTAG_A

Cuts at:      0     384     972 
   Size:        384     588
 
XmnI GAAnn'nnTTC

Cuts at:      0     736     972 
   Size:        736     236
 

 Enzymes that do cut:

     AarI   AflIII     AhdI     AleI    AlwNI     BlpI    BpuEI    BsaAI
    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrGI
    BstXI    BstYI   Bsu36I     BtsI     EaeI     EarI    EcoNI    Hin4I
     MmeI     MscI     MslI     NspI     PciI    SexAI     SfcI     SmlI
    SnaBI     SspI     StyI     TatI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SpeI     SphI     SrfI     StuI     SwaI    TaqII
  Tth111I     XcmI     XhoI     XmaI     ZraI