(Linear) (Six-base) MAPSORT of: KIEE6081.seq  Check: 7090  from: 1  to: 972



KIEE6081.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:22  ..

AfeI AGC'GCT

Cuts at:      0     761     972 
   Size:        761     211
 
AleI CACnn'nnGTG

Cuts at:      0     631     972 
   Size:        631     341
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     393     403     972 
   Size:        393      10     569

  Fragments arranged by size: 

                 569     393      10
 
AlwNI CAG_nnn'CTG

Cuts at:      0     591     972 
   Size:        591     381
 
ApaLI G'TGCA_C

Cuts at:      0     290     972 
   Size:        290     682
 
ApoI r'AATT_y

Cuts at:      0     167     972 
   Size:        167     805
 
AseI AT'TA_AT

Cuts at:      0     152     972 
   Size:        152     820
 
Bme1580I G_kGCm'C

Cuts at:      0     294     972 
   Size:        294     678
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     405     435     972 
   Size:        405      30     537

  Fragments arranged by size: 

                 537     405      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     627     972 
   Size:        627     345
 
BsiHKAI G_wGCw'C

Cuts at:      0     294     972 
   Size:        294     678
 
Bsp1286I G_dGCh'C

Cuts at:      0     294     972 
   Size:        294     678
 
BsrDI GCAATG_nn'

Cuts at:      0     356     972 
   Size:        356     616
 
BsrGI T'GTAC_A

Cuts at:      0     565     972 
   Size:        565     407
 
Bsu36I CC'TnA_GG

Cuts at:      0     690     972 
   Size:        690     282
 
BtsI GCAGTG_nn'

Cuts at:      0     385     830     972 
   Size:        385     445     142

  Fragments arranged by size: 

                 445     385     142
 
DraI TTT'AAA

Cuts at:      0     947     972 
   Size:        947      25
 
EarI CTCTTCn'nnn_

Cuts at:      0     554     972 
   Size:        554     418
 
EcoRV GAT'ATC

Cuts at:      0     540     972 
   Size:        540     432
 
HaeII r_GCGC'y

Cuts at:      0     763     972 
   Size:        763     209
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     407     439     972 
   Size:        407      32     533

  Fragments arranged by size: 

                 533     407      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      24      99     972 
   Size:         24      75     873

  Fragments arranged by size: 

                 873      75      24
 
MslI CAynn'nnrTG

Cuts at:      0     631     670     972 
   Size:        631      39     302

  Fragments arranged by size: 

                 631     302      39
 
MspA1I CmG'CkG

Cuts at:      0     215     972 
   Size:        215     757
 
PfoI T'CCnGG_A

Cuts at:      0     262     972 
   Size:        262     710
 
PvuII CAG'CTG

Cuts at:      0     215     972 
   Size:        215     757
 
SfcI C'TryA_G

Cuts at:      0     221     972 
   Size:        221     751
 
SspI AAT'ATT

Cuts at:      0     150     641     972 
   Size:        150     491     331

  Fragments arranged by size: 

                 491     331     150
 
SwaI ATTT'AAAT

Cuts at:      0     947     972 
   Size:        947      25
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     275     972 
   Size:        275     697
 
TatI w'GTAC_w

Cuts at:      0     457     565     773     972 
   Size:        457     108     208     199

  Fragments arranged by size: 

                 457     208     199     108
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     405     437     972 
   Size:        405      32     535

  Fragments arranged by size: 

                 535     405      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     630     972 
   Size:        630     342
 

 Enzymes that do cut:

     AfeI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI Bme1580I
    BsaXI     BsgI  BsiHKAI Bsp1286I    BsrDI    BsrGI   Bsu36I     BtsI
     DraI     EarI    EcoRV    HaeII    Hin4I     MmeI     MslI   MspA1I
     PfoI    PvuII     SfcI     SspI     SwaI    TaqII     TatI     TstI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AloI     ApaI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI