Restriction map for
pFN21AE6079
(Linear) (Six-base) MAPSORT of: KIEE6079.seq Check: 2423 from: 1 to: 972
KIEE6079.orf
With 182 enzymes: SgfI *
December 5, 2014 13:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 173 972
Size: 173 799
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 699 972
Size: 699 273
AflII C'TTAA_G
Cuts at: 0 129 972
Size: 129 843
AgeI A'CCGG_T
Cuts at: 0 365 972
Size: 365 607
AleI CACnn'nnGTG
Cuts at: 0 25 972
Size: 25 947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 406 972
Size: 406 566
ApaI G_GGCC'C
Cuts at: 0 515 972
Size: 515 457
ApoI r'AATT_y
Cuts at: 0 34 972
Size: 34 938
AvaI C'yCGr_G
Cuts at: 0 928 972
Size: 928 44
BanI G'GyrC_C
Cuts at: 0 442 972
Size: 442 530
BanII G_rGCy'C
Cuts at: 0 104 515 972
Size: 104 411 457
Fragments arranged by size:
457 411 104
BbeI G_GCGC'C
Cuts at: 0 446 972
Size: 446 526
BbsI GAAGACnn'nnnn_
Cuts at: 0 303 972
Size: 303 669
BclI T'GATC_A
Cuts at: 0 521 972
Size: 521 451
BlpI GC'TnA_GC
Cuts at: 0 690 972
Size: 690 282
Bme1580I G_kGCm'C
Cuts at: 0 515 972
Size: 515 457
BmrI ACTGGGnnnn_n'
Cuts at: 0 782 972
Size: 782 190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 972
Size: 533 439
BsaHI Gr'CG_yC
Cuts at: 0 443 972
Size: 443 529
BsaWI w'CCGG_w
Cuts at: 0 365 972
Size: 365 607
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 972
Size: 151 821
BsmI GAATG_Cn'
Cuts at: 0 340 972
Size: 340 632
Bsp1286I G_dGCh'C
Cuts at: 0 104 515 972
Size: 104 411 457
Fragments arranged by size:
457 411 104
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 173 972
Size: 173 799
BsrDI GCAATG_nn'
Cuts at: 0 137 972
Size: 137 835
BsrFI r'CCGG_y
Cuts at: 0 365 972
Size: 365 607
BsrGI T'GTAC_A
Cuts at: 0 776 972
Size: 776 196
BstEII G'GTnAC_C
Cuts at: 0 594 972
Size: 594 378
BstXI CCAn_nnnn'nTGG
Cuts at: 0 591 972
Size: 591 381
BtgI C'CryG_G
Cuts at: 0 851 914 972
Size: 851 63 58
Fragments arranged by size:
851 63 58
DraI TTT'AAA
Cuts at: 0 428 972
Size: 428 544
EarI CTCTTCn'nnn_
Cuts at: 0 757 972
Size: 757 215
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 699 972
Size: 533 166 273
Fragments arranged by size:
533 273 166
EcoO109I rG'GnC_Cy
Cuts at: 0 512 924 972
Size: 512 412 48
Fragments arranged by size:
512 412 48
HaeII r_GCGC'y
Cuts at: 0 446 972
Size: 446 526
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 144 176 972
Size: 144 32 796
Fragments arranged by size:
796 144 32
KasI G'GCGC_C
Cuts at: 0 442 972
Size: 442 530
MfeI C'AATT_G
Cuts at: 0 23 972
Size: 23 949
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 600 972
Size: 600 372
MslI CAynn'nnrTG
Cuts at: 0 25 151 175 778 958 972
Size: 25 126 24 603 180 14
Fragments arranged by size:
603 180 126 25 24 14
NarI GG'CG_CC
Cuts at: 0 443 972
Size: 443 529
NcoI C'CATG_G
Cuts at: 0 914 972
Size: 914 58
NspI r_CATG'y
Cuts at: 0 174 783 972
Size: 174 609 189
Fragments arranged by size:
609 189 174
PfoI T'CCnGG_A
Cuts at: 0 206 972
Size: 206 766
PpuMI rG'GwC_Cy
Cuts at: 0 924 972
Size: 924 48
PspOMI G'GGCC_C
Cuts at: 0 511 972
Size: 511 461
SanDI GG'GwC_CC
Cuts at: 0 924 972
Size: 924 48
ScaI AGT'ACT
Cuts at: 0 304 972
Size: 304 668
SfcI C'TryA_G
Cuts at: 0 5 695 870 972
Size: 5 690 175 102
Fragments arranged by size:
690 175 102 5
SfoI GGC'GCC
Cuts at: 0 444 972
Size: 444 528
SmaI CCC'GGG
Cuts at: 0 930 972
Size: 930 42
SmlI C'TyrA_G
Cuts at: 0 129 972
Size: 129 843
StuI AGG'CCT
Cuts at: 0 712 972
Size: 712 260
StyI C'CwwG_G
Cuts at: 0 839 914 965 972
Size: 839 75 51 7
Fragments arranged by size:
839 75 51 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 442 972
Size: 442 530
TatI w'GTAC_w
Cuts at: 0 179 302 776 972
Size: 179 123 474 196
Fragments arranged by size:
474 196 179 123
XmaI C'CCGG_G
Cuts at: 0 928 972
Size: 928 44
XmnI GAAnn'nnTTC
Cuts at: 0 76 338 972
Size: 76 262 634
Fragments arranged by size:
634 262 76
Enzymes that do cut:
AarI AcuI AflII AgeI AleI AlfI ApaI ApoI
AvaI BanI BanII BbeI BbsI BclI BlpI Bme1580I
BmrI BpmI BsaHI BsaWI BsgI BsmI Bsp1286I BspMI
BsrDI BsrFI BsrGI BstEII BstXI BtgI DraI EarI
Eco57MI EcoO109I HaeII Hin4I KasI MfeI MmeI MslI
NarI NcoI NspI PfoI PpuMI PspOMI SanDI ScaI
SfcI SfoI SmaI SmlI StuI StyI TaqII TatI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AhdI AloI
AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BfrBI BglI BglII BmgBI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI
BseYI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI
BssHII BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MluI MscI MspA1I NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XbaI XcmI
XhoI ZraI