Restriction map for
pFN21AE6076
(Linear) (Six-base) MAPSORT of: KIEE6076.seq Check: 5521 from: 1 to: 969
KIEE6076.orf
With 182 enzymes: SgfI *
December 5, 2014 13:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 587 969
Size: 587 382
AleI CACnn'nnGTG
Cuts at: 0 934 969
Size: 934 35
AlwNI CAG_nnn'CTG
Cuts at: 0 75 694 969
Size: 75 619 275
Fragments arranged by size:
619 275 75
ApaI G_GGCC'C
Cuts at: 0 684 969
Size: 684 285
ApoI r'AATT_y
Cuts at: 0 192 969
Size: 192 777
BanI G'GyrC_C
Cuts at: 0 322 817 969
Size: 322 495 152
Fragments arranged by size:
495 322 152
BanII G_rGCy'C
Cuts at: 0 628 684 969
Size: 628 56 285
Fragments arranged by size:
628 285 56
BlpI GC'TnA_GC
Cuts at: 0 142 695 969
Size: 142 553 274
Fragments arranged by size:
553 274 142
Bme1580I G_kGCm'C
Cuts at: 0 684 969
Size: 684 285
BmrI ACTGGGnnnn_n'
Cuts at: 0 47 631 969
Size: 47 584 338
Fragments arranged by size:
584 338 47
BmtI G_CTAG'C
Cuts at: 0 451 969
Size: 451 518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 969
Size: 226 743
Bpu10I CC'TnA_GC
Cuts at: 0 553 829 969
Size: 553 276 140
Fragments arranged by size:
553 276 140
BsaI GGTCTCn'nnnn_
Cuts at: 0 391 969
Size: 391 578
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 910 969
Size: 148 762 59
Fragments arranged by size:
762 148 59
BsiHKAI G_wGCw'C
Cuts at: 0 143 969
Size: 143 826
BsmI GAATG_Cn'
Cuts at: 0 659 969
Size: 659 310
Bsp1286I G_dGCh'C
Cuts at: 0 143 628 684 969
Size: 143 485 56 285
Fragments arranged by size:
485 285 143 56
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 587 969
Size: 587 382
BsrDI GCAATG_nn'
Cuts at: 0 885 969
Size: 885 84
BstEII G'GTnAC_C
Cuts at: 0 96 969
Size: 96 873
BtsI GCAGTG_nn'
Cuts at: 0 929 969
Size: 929 40
EaeI y'GGCC_r
Cuts at: 0 344 411 488 969
Size: 344 67 77 481
Fragments arranged by size:
481 344 77 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 969
Size: 226 743
EcoNI CCTnn'n_nnAGG
Cuts at: 0 552 969
Size: 552 417
EcoO109I rG'GnC_Cy
Cuts at: 0 465 680 681 969
Size: 465 215 1 288
Fragments arranged by size:
465 288 215 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 781 813 969
Size: 781 32 156
Fragments arranged by size:
781 156 32
HindIII A'AGCT_T
Cuts at: 0 30 969
Size: 30 939
MscI TGG'CCA
Cuts at: 0 413 490 969
Size: 413 77 479
Fragments arranged by size:
479 413 77
MslI CAynn'nnrTG
Cuts at: 0 172 781 934 969
Size: 172 609 153 35
Fragments arranged by size:
609 172 153 35
MspA1I CmG'CkG
Cuts at: 0 146 287 691 969
Size: 146 141 404 278
Fragments arranged by size:
404 278 146 141
NheI G'CTAG_C
Cuts at: 0 447 969
Size: 447 522
PfoI T'CCnGG_A
Cuts at: 0 203 969
Size: 203 766
PpuMI rG'GwC_Cy
Cuts at: 0 465 969
Size: 465 504
PspOMI G'GGCC_C
Cuts at: 0 680 969
Size: 680 289
PvuII CAG'CTG
Cuts at: 0 146 287 691 969
Size: 146 141 404 278
Fragments arranged by size:
404 278 146 141
SfcI C'TryA_G
Cuts at: 0 686 969
Size: 686 283
SpeI A'CTAG_T
Cuts at: 0 429 969
Size: 429 540
StyI C'CwwG_G
Cuts at: 0 911 969
Size: 911 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 589 969
Size: 589 380
TatI w'GTAC_w
Cuts at: 0 176 969
Size: 176 793
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 483 969
Size: 483 486
XmnI GAAnn'nnTTC
Cuts at: 0 859 969
Size: 859 110
Enzymes that do cut:
AarI AleI AlwNI ApaI ApoI BanI BanII BlpI
Bme1580I BmrI BmtI BpmI Bpu10I BsaI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrDI BstEII BtsI EaeI Eco57MI
EcoNI EcoO109I Hin4I HindIII MscI MslI MspA1I NheI
PfoI PpuMI PspOMI PvuII SfcI SpeI StyI TaqII
TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BmgBI BplI BpuEI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI
ZraI