Restriction map for
pFN21AE6074
(Linear) (Six-base) MAPSORT of: KIEE6074.seq Check: 2900 from: 1 to: 969
KIEE6074.orf
With 182 enzymes: SgfI *
December 5, 2014 13:22 ..
AccI GT'mk_AC
Cuts at: 0 103 521 969
Size: 103 418 448
Fragments arranged by size:
448 418 103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 671 969
Size: 2 669 298
Fragments arranged by size:
669 298 2
AlwNI CAG_nnn'CTG
Cuts at: 0 287 969
Size: 287 682
ApaLI G'TGCA_C
Cuts at: 0 260 460 969
Size: 260 200 509
Fragments arranged by size:
509 260 200
ApoI r'AATT_y
Cuts at: 0 31 299 611 805 929 969
Size: 31 268 312 194 124 40
Fragments arranged by size:
312 268 194 124 40 31
AvaI C'yCGr_G
Cuts at: 0 545 969
Size: 545 424
BbsI GAAGACnn'nnnn_
Cuts at: 0 677 969
Size: 677 292
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 401 435 969
Size: 401 34 534
Fragments arranged by size:
534 401 34
Bme1580I G_kGCm'C
Cuts at: 0 264 464 969
Size: 264 200 505
Fragments arranged by size:
505 264 200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 344 969
Size: 52 292 625
Fragments arranged by size:
625 292 52
Bpu10I CC'TnA_GC
Cuts at: 0 622 969
Size: 622 347
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 969
Size: 664 305
BsaI GGTCTCn'nnnn_
Cuts at: 0 262 969
Size: 262 707
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 777 807 969
Size: 777 30 162
Fragments arranged by size:
777 162 30
BseYI C'CCAG_C
Cuts at: 0 117 969
Size: 117 852
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 969
Size: 148 821
BsiHKAI G_wGCw'C
Cuts at: 0 264 464 969
Size: 264 200 505
Fragments arranged by size:
505 264 200
Bsp1286I G_dGCh'C
Cuts at: 0 264 464 969
Size: 264 200 505
Fragments arranged by size:
505 264 200
BspHI T'CATG_A
Cuts at: 0 404 969
Size: 404 565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 503 969
Size: 503 466
BsrDI GCAATG_nn'
Cuts at: 0 701 969
Size: 701 268
BssSI C'ACGA_G
Cuts at: 0 265 969
Size: 265 704
BstZ17I GTA'TAC
Cuts at: 0 104 969
Size: 104 865
Bsu36I CC'TnA_GG
Cuts at: 0 916 969
Size: 916 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 52 344 671 969
Size: 2 50 292 327 298
Fragments arranged by size:
327 298 292 50 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 360 969
Size: 360 609
EcoRI G'AATT_C
Cuts at: 0 611 969
Size: 611 358
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 746 778 969
Size: 259 32 455 32 191
Fragments arranged by size:
455 259 191 32 32
HincII GTy'rAC
Cuts at: 0 207 969
Size: 207 762
MslI CAynn'nnrTG
Cuts at: 0 772 969
Size: 772 197
MspA1I CmG'CkG
Cuts at: 0 691 969
Size: 691 278
NdeI CA'TA_TG
Cuts at: 0 376 969
Size: 376 593
NspI r_CATG'y
Cuts at: 0 422 969
Size: 422 547
PshAI GACnn'nnGTC
Cuts at: 0 212 969
Size: 212 757
PspXI vC'TCGA_Gb
Cuts at: 0 545 969
Size: 545 424
PvuII CAG'CTG
Cuts at: 0 691 969
Size: 691 278
SfcI C'TryA_G
Cuts at: 0 51 969
Size: 51 918
SmlI C'TyrA_G
Cuts at: 0 545 679 969
Size: 545 134 290
Fragments arranged by size:
545 290 134
SpeI A'CTAG_T
Cuts at: 0 945 969
Size: 945 24
SspI AAT'ATT
Cuts at: 0 743 969
Size: 743 226
StyI C'CwwG_G
Cuts at: 0 922 969
Size: 922 47
TatI w'GTAC_w
Cuts at: 0 36 176 969
Size: 36 140 793
Fragments arranged by size:
793 140 36
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 18 795 969
Size: 18 777 174
Fragments arranged by size:
777 174 18
XhoI C'TCGA_G
Cuts at: 0 545 969
Size: 545 424
XmnI GAAnn'nnTTC
Cuts at: 0 195 615 969
Size: 195 420 354
Fragments arranged by size:
420 354 195
Enzymes that do cut:
AccI AcuI AlwNI ApaLI ApoI AvaI BbsI BcgI
Bme1580I BpmI Bpu10I BpuEI BsaI BsaXI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrDI BssSI BstZ17I Bsu36I
Eco57MI EcoNI EcoRI Hin4I HincII MslI MspA1I NdeI
NspI PshAI PspXI PvuII SfcI SmlI SpeI SspI
StyI TatI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI
BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI EcoO109I EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SphI SrfI StuI
SwaI TaqII TstI Tth111I XbaI XmaI ZraI