Restriction map for
pFN21AE6071
(Linear) (Six-base) MAPSORT of: KIEE6071.seq Check: 5483 from: 1 to: 963
KIEE6071.orf
With 182 enzymes: SgfI *
December 5, 2014 13:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 806 963
Size: 88 718 157
Fragments arranged by size:
718 157 88
AleI CACnn'nnGTG
Cuts at: 0 115 963
Size: 115 848
ApoI r'AATT_y
Cuts at: 0 31 249 518 945 963
Size: 31 218 269 427 18
Fragments arranged by size:
427 269 218 31 18
AseI AT'TA_AT
Cuts at: 0 142 581 963
Size: 142 439 382
Fragments arranged by size:
439 382 142
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 376 409 794 827 963
Size: 376 33 385 33 136
Fragments arranged by size:
385 376 136 33 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 76 154 754 963
Size: 76 78 600 209
Fragments arranged by size:
600 209 78 76
Bpu10I CC'TnA_GC
Cuts at: 0 21 963
Size: 21 942
BsaBI GATnn'nnATC
Cuts at: 0 598 963
Size: 598 365
BsmI GAATG_Cn'
Cuts at: 0 422 462 963
Size: 422 40 501
Fragments arranged by size:
501 422 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 467 497 963
Size: 467 30 466
Fragments arranged by size:
467 466 30
BsrDI GCAATG_nn'
Cuts at: 0 629 963
Size: 629 334
BsrGI T'GTAC_A
Cuts at: 0 176 565 963
Size: 176 389 398
Fragments arranged by size:
398 389 176
BstEII G'GTnAC_C
Cuts at: 0 108 963
Size: 108 855
BstXI CCAn_nnnn'nTGG
Cuts at: 0 105 963
Size: 105 858
BstYI r'GATC_y
Cuts at: 0 570 963
Size: 570 393
BtgI C'CryG_G
Cuts at: 0 113 963
Size: 113 850
BtsI GCAGTG_nn'
Cuts at: 0 385 963
Size: 385 578
EaeI y'GGCC_r
Cuts at: 0 371 963
Size: 371 592
EarI CTCTTCn'nnn_
Cuts at: 0 693 963
Size: 693 270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 806 963
Size: 88 718 157
Fragments arranged by size:
718 157 88
EcoO109I rG'GnC_Cy
Cuts at: 0 440 963
Size: 440 523
EcoRI G'AATT_C
Cuts at: 0 31 963
Size: 31 932
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 515 963
Size: 515 448
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 636 668 963
Size: 636 32 295
Fragments arranged by size:
636 295 32
HincII GTy'rAC
Cuts at: 0 497 963
Size: 497 466
HpaI GTT'AAC
Cuts at: 0 497 963
Size: 497 466
MscI TGG'CCA
Cuts at: 0 373 963
Size: 373 590
MslI CAynn'nnrTG
Cuts at: 0 115 172 403 427 963
Size: 115 57 231 24 536
Fragments arranged by size:
536 231 115 57 24
MspA1I CmG'CkG
Cuts at: 0 28 963
Size: 28 935
NcoI C'CATG_G
Cuts at: 0 113 963
Size: 113 850
NdeI CA'TA_TG
Cuts at: 0 376 963
Size: 376 587
PsiI TTA'TAA
Cuts at: 0 914 963
Size: 914 49
PstI C_TGCA'G
Cuts at: 0 392 508 963
Size: 392 116 455
Fragments arranged by size:
455 392 116
PvuII CAG'CTG
Cuts at: 0 28 963
Size: 28 935
SfcI C'TryA_G
Cuts at: 0 388 504 938 963
Size: 388 116 434 25
Fragments arranged by size:
434 388 116 25
SspI AAT'ATT
Cuts at: 0 749 818 963
Size: 749 69 145
Fragments arranged by size:
749 145 69
StyI C'CwwG_G
Cuts at: 0 113 689 963
Size: 113 576 274
Fragments arranged by size:
576 274 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 398 963
Size: 398 565
TatI w'GTAC_w
Cuts at: 0 176 565 963
Size: 176 389 398
Fragments arranged by size:
398 389 176
XmnI GAAnn'nnTTC
Cuts at: 0 273 963
Size: 273 690
Enzymes that do cut:
AcuI AleI ApoI AseI BaeI BbsI Bpu10I BsaBI
BsmI BspMI BsrDI BsrGI BstEII BstXI BstYI BtgI
BtsI EaeI EarI Eco57MI EcoO109I EcoRI FalI Hin4I
HincII HpaI MscI MslI MspA1I NcoI NdeI PsiI
PstI PvuII SfcI SspI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV
FseI FspI FspAI HaeII HindIII KasI KpnI MfeI
MluI MmeI NaeI NarI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI